Detailed information of TA system TA233164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 12121..12772
• Replicon: plasmid p1
• Accession: NZ_CP091329
• Organism: Acinetobacter baumannii strain BM2333

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: L3Q73_RS19810
• Protein ID: WP_000269904.1
• Coordinates: 12121..12477 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L3Q73_RS19815
• Protein ID: WP_001129974.1
• Coordinates: 12470..12772 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Q73_RS19790 (L3Q73_19790)	7749..8918	+	1170	WP_002134206.1	MobA/MobL family protein	-
L3Q73_RS19795 (L3Q73_19795)	9515..10450	+	936	WP_001292329.1	replication initiation protein RepM	-
L3Q73_RS19800 (L3Q73_19800)	10528..11046	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
L3Q73_RS19805 (L3Q73_19805)	11256..11555	+	300	WP_000358348.1	hypothetical protein	-
L3Q73_RS19810 (L3Q73_19810)	12121..12477	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3Q73_RS19815 (L3Q73_19815)	12470..12772	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
L3Q73_RS19820 (L3Q73_19820)	12765..12974	+	210	WP_000069474.1	hypothetical protein	-
L3Q73_RS19825 (L3Q73_19825)	13300..13761	-	462	WP_000761346.1	DIP1984 family protein	-
L3Q73_RS19830 (L3Q73_19830)	14066..16477	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
L3Q73_RS19835 (L3Q73_19835)	16605..16919	-	315	WP_001983304.1	BrnA antitoxin family protein	-
L3Q73_RS19840 (L3Q73_19840)	16906..17193	-	288	WP_000438827.1	BrnT family toxin	-
L3Q73_RS19845 (L3Q73_19845)	17461..17760	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22388	22388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T233164 WP_000269904.1 NZ_CP091329:12121-12477 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T233164 NZ_CP128548:c4863140-4862844 [Pseudomonas kurunegalensis]
ATGAGCGAAAAACTGATTCCGTTTGATATGGCAGCTTTGTTGAGTAGCGATGAGGCCATCAGTGAGTACCTTAGCCAGGT
GCTATCAGATGGCGATACCGAAGAGATCATTCGAGCGCTCGGCCATATCGCGCGTGCACGAGGCATGACACACATAGCCA
CGGAGAGTGGTTTGGGCAGAGAAAGCCTCTACAAGGCGCTCAGCCCAGGCGCAAAGCCTCGTTTTGACACTGTGTTGAAG
GTCATTCGAGCCTTGGGGGTAGACCTTCTCGTGCAGCCCCATGCGGTAGCCCGTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT233164 WP_001129974.1 NZ_CP091329:12470-12772 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT233164 NZ_CP128548:c4863430-4863137 [Pseudomonas kurunegalensis]
ATGTATCTTGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGGGATTTGAAAGCCAAGCTTGCGATAGG
TCGCAGGCTGGAGCGAGTTGCGATAGGCAACCTTGGTGACTTCAAGTGGCTTGGAGGCGGTCTAGGTGAGCTTCGCATCG
ACGTAGGTGCAGGCTATCGCGTCTATTTCACGCAAAAGGGGCACAGATTGGTTCTGCTGCTGGTTGGCGGCGACAAGTCC
ACTCAAGTCGCCGATATTCTCAAATCAAGAAAGCTGCTGAAGGAGATGATATGA

Structures

Toxin

• AlphaFold DB: N8YFZ1