Detailed information of TA system TA233162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 927..1578
• Replicon: plasmid p1
• Accession: NZ_CP091329
• Organism: Acinetobacter baumannii strain BM2333

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: L3Q73_RS19745
• Protein ID: WP_000269904.1
• Coordinates: 927..1283 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L3Q73_RS19750
• Protein ID: WP_001129974.1
• Coordinates: 1276..1578 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Q73_RS19740 (L3Q73_19740)	62..361	+	300	WP_000358348.1	hypothetical protein	-
L3Q73_RS19745 (L3Q73_19745)	927..1283	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3Q73_RS19750 (L3Q73_19750)	1276..1578	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
L3Q73_RS19755 (L3Q73_19755)	1571..1780	+	210	WP_000069474.1	hypothetical protein	-
L3Q73_RS19760 (L3Q73_19760)	2106..2567	-	462	WP_000761346.1	DIP1984 family protein	-
L3Q73_RS19765 (L3Q73_19765)	2872..5283	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
L3Q73_RS19770 (L3Q73_19770)	5411..5725	-	315	WP_001983304.1	BrnA antitoxin family protein	-
L3Q73_RS19775 (L3Q73_19775)	5712..5999	-	288	WP_000438827.1	BrnT family toxin	-
L3Q73_RS19780 (L3Q73_19780)	6267..6566	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22388	22388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T233162 WP_000269904.1 NZ_CP091329:927-1283 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T233162 NZ_CP128548:c4856802-4856494 [Pseudomonas kurunegalensis]
ATGGGTAACGTCAAGTTGAAAAAATGGGACAGTGCCGAACACCTCAAGACGGACGAGGACATGGCGCTTTACCTCGAAGC
TTGCATTGAAGAGGCGGGTGATGATGCGGCTTTTATCGCCAAGGCGCTCGGCACCATTGCGCGTGCAAAAGGCATGGCTC
AGTTGGCCCGTGATACCGGGCTTGGGCGTGAAAGCCTGTACAAGGCGTTGTCCGGCGAGGGGAACCCAAGCTTCGCCACC
ATCCTGAAGGTGACCAGAGCGCTTGGCCTACAGCTTCATGCTGACGTTGCATCCACTCATACTGGCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT233162 WP_001129974.1 NZ_CP091329:1276-1578 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT233162 NZ_CP128548:c4857102-4856812 [Pseudomonas kurunegalensis]
GTGAAGACCATCCTCACAACCGATGATTTCGATGCTTGGTTTTCCAAGCTTAGCGATAAGCGATCTGCGATGCGCATCCA
GGCGCGCATCGACCGTGCCGAAGCAGGGAATTTCGGTGATTGCCAAGCTGTGGGTGAGGGCGTCTCCGAGATGCGTATTC
ACTATGGGCCAGGATATCGTGTGTACTTCACCCAGCGCGGCCTTGAAATCGTGATCCTGCTGGCGGGTGGCGATAAGTCC
ACTCAATCAAAAGACATCAAAACCGCCCTCGACATTGCACGACGGCTTTAG

Structures

Toxin

• AlphaFold DB: N8YFZ1