Detailed information of TA system TA233152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3836488..3837134
• Replicon: chromosome
• Accession: NZ_CP091326
• Organism: Klebsiella pneumoniae strain hvK2115

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L3Y29_RS18880
• Protein ID: WP_159147099.1
• Coordinates: 3836787..3837134 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A231WWU8
• Locus tag: L3Y29_RS18875
• Protein ID: WP_004174017.1
• Coordinates: 3836488..3836787 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y29_RS18855 (3832694)	3832694..3833452	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
L3Y29_RS18860 (3833502)	3833502..3834332	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
L3Y29_RS18865 (3834383)	3834383..3834712	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
L3Y29_RS18870 (3834917)	3834917..3836425	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
L3Y29_RS18875 (3836488)	3836488..3836787	-	300	WP_004174017.1	XRE family transcriptional regulator	Antitoxin
L3Y29_RS18880 (3836787)	3836787..3837134	-	348	WP_159147099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3Y29_RS18885 (3837302)	3837302..3839749	-	2448	WP_029884517.1	glycogen phosphorylase	-
L3Y29_RS18890 (3839767)	3839767..3841200	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13590.61 Da
• Isoelectric Point: 5.6802

>T233152 WP_159147099.1 NZ_CP091326:c3837134-3836787 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHELPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T233152 NZ_CP128545:c4700471-4700184 [Pseudomonas monteilii]
ATGACCTATAGCCTCGACTTCGATTGCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACAGTGCGTGAGCAGTTCAA
GAAGAAACTGGCAGAGGTCTTGCTCAATCCTCGAGTCGAGGCAAACCGCCTTCACTCGCTGCCAGATTGCTACAAAATCA
AGCTTCGCAGCAGTGGGTACAGGCTGGTCTATCAGGTCATTGGCCAAGAGATCGTGGTTTTTGTCGTTGCTGTTGATCGC
CGTGAACGGGACCAAGCTTACAAAAAGGCTGCTGAGCGGCTTGAATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10844.64 Da
• Isoelectric Point: 10.1464

>AT233152 WP_004174017.1 NZ_CP091326:c3836787-3836488 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT233152 NZ_CP128545:c4700700-4700461 [Pseudomonas monteilii]
ATGGCTTCCATCAATATCCGCGTCGACGACGATCTCAAAGCTCGCGCCTACAAAGAGCTTGAGCGTCTTGGTGTAACCCC
CTCCGAACTCATGCGCCAAGCCCTGCAATATGTAGCTGAGCGGGGCAAGCTACCCTTCCGGCCGGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCCACTGTACAAGAGAGGCTTGCTTCCCCGCAGCGCGTGAGGGTTCAGCTGGATGACCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A231WWU8