Detailed information of TA system TA233137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4631898..4632500
• Replicon: chromosome
• Accession: NZ_CP091319
• Organism: Enterobacter sp. JH25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: L2Y28_RS22215
• Protein ID: WP_022649837.1
• Coordinates: 4632189..4632500 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2Y28_RS22210
• Protein ID: WP_022649836.1
• Coordinates: 4631898..4632188 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y28_RS22195	4629396..4630298	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
L2Y28_RS22200	4630295..4630930	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
L2Y28_RS22205	4630927..4631856	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
L2Y28_RS22210	4631898..4632188	-	291	WP_022649836.1	NadS family protein	Antitoxin
L2Y28_RS22215	4632189..4632500	-	312	WP_022649837.1	hypothetical protein	Toxin
L2Y28_RS22220	4632649..4633590	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
L2Y28_RS22225	4633635..4634072	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
L2Y28_RS22230	4634069..4634950	-	882	WP_022649840.1	virulence factor BrkB family protein	-
L2Y28_RS22235	4634944..4635543	-	600	WP_022649841.1	glucose-1-phosphatase	-
L2Y28_RS22240	4635662..4636462	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
L2Y28_RS22245	4636497..4637393	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4621330..4632500	11170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T233137 WP_022649837.1 NZ_CP091319:c4632500-4632189 [Enterobacter sp. JH25]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T233137 NZ_CP128541:5256532-5256999 [Pseudomonas monteilii]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
GGCACTCAGCGCTCAAGTGCAGGCTCAGATGCACAAGGACCCAACGGGTTACACGCGCAGAAATGCCTATAAAAGGCTGG
CGGCCATCAAGCGACTGGCCTTCGATGTAATCCCTCAAGACCCGACCAAACCTGAGTACCGGCAGGGGGCCACACTGGGT
GGTGACCACAAGCACTGGTTCAGGGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTATCACACCCCCAGCAGGAT
CATTGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTACGAAAGTAGCGACGATGCTTACAAGGTCTTTCAGA
AGATGCTGCATACCGGCCACCCGCCGGACGATTGGGAGCAGCTACTGCGAGAAGCTGCAGCCGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT233137 WP_022649836.1 NZ_CP091319:c4632188-4631898 [Enterobacter sp. JH25]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT233137 NZ_CP128541:5256209-5256535 [Pseudomonas monteilii]
ATGGCAACCACTCTCGACGTCGAGTCCACACTGACCGATCGCTATCAGACCACTGTTCCCGAAACAGTACGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCTGACGGTGAGGTGATACTCACCCGTGCAGAAGCAACCG
AGAGCGATCCAGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCCACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGACCTCATCGCGCGTCTCGACAAGCTTGTCGGCGACGCAGAGGTCGACCTGGATCAATCTCTGTCGGCGGATGA
TGAATGA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6