Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1976792..1977405 Replicon chromosome
Accession NZ_CP091318
Organism Methylocystis sp. SB2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LNB28_RS09730 Protein ID WP_029652175.1
Coordinates 1976792..1977088 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LNB28_RS09735 Protein ID WP_029652176.1
Coordinates 1977097..1977405 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNB28_RS09710 (LNB28_09710) 1973073..1974119 + 1047 WP_029652170.1 class I SAM-dependent methyltransferase -
LNB28_RS09715 (LNB28_09715) 1974179..1974613 - 435 WP_239753671.1 cupin domain-containing protein -
LNB28_RS09720 (LNB28_09720) 1974694..1975395 - 702 WP_029652172.1 VIT family protein -
LNB28_RS09725 (LNB28_09725) 1975434..1976549 - 1116 WP_055725734.1 SbmA/BacA-like family transporter -
LNB28_RS09730 (LNB28_09730) 1976792..1977088 + 297 WP_029652175.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNB28_RS09735 (LNB28_09735) 1977097..1977405 + 309 WP_029652176.1 HigA family addiction module antitoxin Antitoxin
LNB28_RS09745 (LNB28_09745) 1977676..1977993 + 318 WP_026222665.1 hypothetical protein -
LNB28_RS09750 (LNB28_09750) 1978026..1978673 + 648 WP_029652177.1 peptide-methionine (S)-S-oxide reductase MsrA -
LNB28_RS09755 (LNB28_09755) 1978788..1979780 - 993 WP_083481415.1 penicillin-insensitive murein endopeptidase -
LNB28_RS09760 (LNB28_09760) 1979938..1980489 + 552 WP_029651806.1 L,D-transpeptidase -
LNB28_RS09765 (LNB28_09765) 1980590..1981630 + 1041 WP_029651807.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11695.17 Da        Isoelectric Point: 6.4709

>T233121 WP_029652175.1 NZ_CP091318:1976792-1977088 [Methylocystis sp. SB2]
MIESFRDDWLRAFFVDDVRSKKIPPDLESRLFRKLQMIDDATTDQDLRVPPSNHFEKLRGDLADFYSIRVNKRWRLIFQW
DGAKGEASGVYLDNHSYQ

Download         Length: 297 bp

>T233121 NZ_CP128532:290210-290479 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11598.62 Da        Isoelectric Point: 10.9969

>AT233121 WP_029652176.1 NZ_CP091318:1977097-1977405 [Methylocystis sp. SB2]
MLMTKRKPVTVAEILVEEFMEPMGLTQAALAAAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
EAMHSPRERKRIERAKPLKAVA

Download         Length: 309 bp

>AT233121 NZ_CP128532:289957-290223 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References