Detailed information of TA system TA023312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2806351..2807045
• Replicon: chromosome
• Accession: NC_011740
• Organism: Escherichia fergusonii ATCC 35469

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: B7LNG9
• Locus tag: EFER_RS13850
• Protein ID: WP_001263502.1
• Coordinates: 2806351..2806749 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EFER_RS13855
• Protein ID: WP_000554758.1
• Coordinates: 2806752..2807045 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFER_RS13820	2801479..2802936	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
EFER_RS13825	2802984..2803226	+	243	WP_001029361.1	hypothetical protein	-
EFER_RS13830	2803235..2803759	-	525	WP_024256511.1	hydrolase	-
EFER_RS13835	2803821..2804435	-	615	WP_000602110.1	peptide chain release factor H	-
EFER_RS13840	2804432..2805571	-	1140	WP_000521571.1	RNA ligase RtcB family protein	-
EFER_RS13845	2805889..2806341	-	453	WP_001059849.1	GNAT family N-acetyltransferase	-
EFER_RS13850	2806351..2806749	-	399	WP_001263502.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EFER_RS13855	2806752..2807045	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EFER_RS13860	2807097..2808152	-	1056	WP_001226166.1	DNA polymerase IV	-
EFER_RS13865	2808223..2809008	-	786	WP_000207582.1	putative lateral flagellar export/assembly protein LafU	-
EFER_RS13870	2808989..2810692	+	1704	Protein_2624	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15486.90 Da
• Isoelectric Point: 8.9401

>T23312 WP_001263502.1 NC_011740:c2806749-2806351 [Escherichia fergusonii ATCC 35469]
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T23312 NC_011740:c2806749-2806351 [Escherichia fergusonii ATCC 35469]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCCAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT23312 WP_000554758.1 NC_011740:c2807045-2806752 [Escherichia fergusonii ATCC 35469]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT23312 NC_011740:c2807045-2806752 [Escherichia fergusonii ATCC 35469]
ATGCATCGAATTCTTGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: B7LNG9

Antitoxin

• AlphaFold DB: S1QAE3