Detailed information of TA system TA233113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1937095..1937688
• Replicon: chromosome
• Accession: NZ_CP091308
• Organism: Burkholderia pyrrocinia strain LWK2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L2Y90_RS26310
• Protein ID: WP_258283240.1
• Coordinates: 1937410..1937688 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2Y90_RS26305
• Protein ID: WP_034185116.1
• Coordinates: 1937095..1937397 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y90_RS26280 (L2Y90_26225)	1932246..1933517	+	1272	WP_152855722.1	anthranilate 1,2-dioxygenase large subunit AndAc	-
L2Y90_RS26285 (L2Y90_26230)	1933532..1934008	+	477	WP_131948025.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L2Y90_RS26290 (L2Y90_26235)	1934026..1934352	+	327	WP_048022186.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L2Y90_RS26295 (L2Y90_26240)	1934342..1935562	+	1221	WP_258283238.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L2Y90_RS26300 (L2Y90_26245)	1935775..1937061	+	1287	WP_258283239.1	DUF445 domain-containing protein	-
L2Y90_RS26305 (L2Y90_26250)	1937095..1937397	-	303	WP_034185116.1	HigA family addiction module antitoxin	Antitoxin
L2Y90_RS26310 (L2Y90_26255)	1937410..1937688	-	279	WP_258283240.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Y90_RS26315 (L2Y90_26260)	1938054..1938353	-	300	WP_034185118.1	putative addiction module antidote protein	-
L2Y90_RS26320 (L2Y90_26265)	1938357..1938674	-	318	WP_258283241.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L2Y90_RS26325 (L2Y90_26270)	1939023..1940663	+	1641	WP_152855717.1	acetolactate synthase large subunit	-
L2Y90_RS26330 (L2Y90_26275)	1940693..1942126	+	1434	WP_258283242.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10655.12 Da
• Isoelectric Point: 8.5478

>T233113 WP_258283240.1 NZ_CP091308:c1937688-1937410 [Burkholderia pyrrocinia]
MIRSFRCKDTQLLFAGASPRRFRTIETVASRKLQMVAAAKELRDLRAPPGNRLEALQGDREGQHGIRINEQWRICFEWSD
DGPTQVEIVDYH

Nucleotide

• Length: 279 bp

>T233113 NZ_CP128531:c194550-194218 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11570.36 Da
• Isoelectric Point: 8.5562

>AT233113 WP_034185116.1 NZ_CP091308:c1937397-1937095 [Burkholderia pyrrocinia]
MLKNGMRPIHPGEILREEFLVPLEMSVNALALALNVPATRLHEIVKERRGITADTAYRLARYFDTSPEFWLNLQAAYDLK
TLPTRSEIDRKVEPRERTHA

Nucleotide

• Length: 303 bp

>AT233113 NZ_CP128531:c194807-194547 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCATGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA