Detailed information of TA system TA233101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 121932..122467
• Replicon: plasmid pUK043_1
• Accession: NZ_CP091302
• Organism: Enterococcus faecium strain UK043

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: L3K57_RS14595
• Protein ID: WP_002314366.1
• Coordinates: 122195..122467 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: L3K57_RS14590
• Protein ID: WP_236918134.1
• Coordinates: 121932..122195 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3K57_RS14575 (L3K57_14575)	117360..119609	+	2250	WP_002314387.1	alpha-galactosidase	-
L3K57_RS14580 (L3K57_14580)	119612..120787	+	1176	WP_002336205.1	MFS transporter	-
L3K57_RS14585 (L3K57_14585)	120790..121698	-	909	Protein_129	IS982-like element ISEfm1 family transposase	-
L3K57_RS14590 (L3K57_14590)	121932..122195	+	264	WP_236918134.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L3K57_RS14595 (L3K57_14595)	122195..122467	+	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
L3K57_RS14600 (L3K57_14600)	122544..122873	+	330	WP_002292678.1	AbrB family transcriptional regulator	-
L3K57_RS14605 (L3K57_14605)	122863..123149	+	287	Protein_133	type II toxin-antitoxin system RelE/ParE family toxin	-
L3K57_RS14610 (L3K57_14610)	123434..124291	+	858	WP_002292680.1	ParA family protein	-
L3K57_RS14615 (L3K57_14615)	124292..124840	+	549	WP_002292681.1	hypothetical protein	-
L3K57_RS14620 (L3K57_14620)	125100..125477	+	378	WP_010729388.1	hypothetical protein	-
L3K57_RS14625 (L3K57_14625)	125511..126191	-	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
L3K57_RS14630 (L3K57_14630)	126267..126910	-	644	Protein_138	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..161029	161029
	-	inside	IScluster/Tn	-	-	111331..135890	24559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (6-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T233101 WP_002314366.1 NZ_CP091302:122195-122467 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T233101 NZ_CP128529:1519818-1520087 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9989.24 Da
• Isoelectric Point: 4.4066

>AT233101 WP_236918134.1 NZ_CP091302:121932-122195 [Enterococcus faecium]
MAKTVLNPSSAWKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT233101 NZ_CP128529:1519548-1519814 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A829F704