Detailed information of TA system TA233074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1263729..1264293
• Replicon: chromosome
• Accession: NZ_CP091289
• Organism: Lacticaseibacillus paracasei subsp. paracasei strain GR0562

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A6G9EML4
• Locus tag: L3C06_RS06080
• Protein ID: WP_014951859.1
• Coordinates: 1263991..1264293 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: L3C06_RS06075
• Protein ID: WP_236360678.1
• Coordinates: 1263729..1263998 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3C06_RS06045 (L3C06_06045)	1259070..1259957	-	888	WP_016385537.1	ABC transporter ATP-binding protein	-
L3C06_RS06050 (L3C06_06050)	1260154..1261380	-	1227	WP_003574766.1	hypothetical protein	-
L3C06_RS06055 (L3C06_06055)	1261578..1261982	+	405	WP_003574768.1	MerR family transcriptional regulator	-
L3C06_RS06060 (L3C06_06060)	1261975..1262301	+	327	WP_003658351.1	YrdB family protein	-
L3C06_RS06065 (L3C06_06065)	1262363..1262657	+	295	Protein_1170	hypothetical protein	-
L3C06_RS06070 (L3C06_06070)	1262702..1263355	+	654	WP_003570049.1	N-acetyltransferase	-
L3C06_RS06075 (L3C06_06075)	1263729..1263998	+	270	WP_236360678.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
L3C06_RS06080 (L3C06_06080)	1263991..1264293	+	303	WP_014951859.1	type II toxin-antitoxin system YafQ family toxin	Toxin
L3C06_RS06085 (L3C06_06085)	1264632..1264775	+	144	WP_003574774.1	hypothetical protein	-
L3C06_RS06090 (L3C06_06090)	1265002..1265589	+	588	WP_236360679.1	hypothetical protein	-
L3C06_RS06095 (L3C06_06095)	1265825..1268002	+	2178	WP_016385304.1	Tex family protein	-
L3C06_RS06100 (L3C06_06100)	1268177..1268614	-	438	WP_016385305.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-98)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11409.21 Da
• Isoelectric Point: 10.2818

>T233074 WP_014951859.1 NZ_CP091289:1263991-1264293 [Lacticaseibacillus paracasei subsp. paracasei]
VYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T233074 NZ_CP128526:1314683-1314859 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9955.66 Da
• Isoelectric Point: 7.2026

>AT233074 WP_236360678.1 NZ_CP091289:1263729-1263998 [Lacticaseibacillus paracasei subsp. paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLSV

Nucleotide

• Length: 270 bp

>AT233074 NZ_CP128526:1314915-1315331 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6G9EML4