Detailed information of TA system TA233065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2344740..2345293
• Replicon: chromosome
• Accession: NZ_CP091244
• Organism: Thiothrix winogradskyi strain CT3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L2Y54_RS11970
• Protein ID: WP_236496340.1
• Coordinates: 2344740..2345018 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2Y54_RS11975
• Protein ID: WP_236496341.1
• Coordinates: 2345030..2345293 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y54_RS11945 (L2Y54_11945)	2341733..2342374	-	642	WP_236496336.1	7-carboxy-7-deazaguanine synthase	-
L2Y54_RS11950 (L2Y54_11950)	2342371..2343459	-	1089	WP_236496337.1	6-carboxytetrahydropterin synthase	-
L2Y54_RS11955 (L2Y54_11955)	2343600..2343986	-	387	WP_236496338.1	type II toxin-antitoxin system VapC family toxin	-
L2Y54_RS11960 (L2Y54_11960)	2343983..2344207	-	225	WP_210225821.1	DUF2281 domain-containing protein	-
L2Y54_RS11965 (L2Y54_11965)	2344299..2344583	-	285	WP_236496339.1	6-carboxytetrahydropterin synthase	-
L2Y54_RS11970 (L2Y54_11970)	2344740..2345018	+	279	WP_236496340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Y54_RS11975 (L2Y54_11975)	2345030..2345293	+	264	WP_236496341.1	HigA family addiction module antitoxin	Antitoxin
L2Y54_RS11980 (L2Y54_11980)	2345296..2345577	-	282	WP_236496342.1	DUF2283 domain-containing protein	-
L2Y54_RS11985 (L2Y54_11985)	2345849..2346550	-	702	WP_236496343.1	competence protein CoiA family protein	-
L2Y54_RS11990 (L2Y54_11990)	2346713..2346955	-	243	WP_236496344.1	ankyrin repeat domain-containing protein	-
L2Y54_RS11995 (L2Y54_11995)	2347038..2347454	+	417	WP_236496345.1	hypothetical protein	-
L2Y54_RS12000 (L2Y54_12000)	2347498..2347905	+	408	WP_236496346.1	hypothetical protein	-
L2Y54_RS12005 (L2Y54_12005)	2348027..2348179	-	153	WP_236496347.1	hypothetical protein	-
L2Y54_RS12010 (L2Y54_12010)	2348159..2348638	+	480	WP_236496348.1	hypothetical protein	-
L2Y54_RS12015 (L2Y54_12015)	2348638..2348853	+	216	WP_236496349.1	hypothetical protein	-
L2Y54_RS12020 (L2Y54_12020)	2349513..2350112	-	600	WP_236496350.1	Uma2 family endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10698.18 Da
• Isoelectric Point: 6.4788

>T233065 WP_236496340.1 NZ_CP091244:2344740-2345018 [Thiothrix winogradskyi]
MIISFIHKGLERFYLTGSKAGIQPEHAPKLRRILTVLETAQIPDDMDLPGYRLHPLQGNRQGTWSVRVSGNWLITFQFTE
QNVELVDYEDYH

Nucleotide

• Length: 279 bp

>T233065 NZ_CP128525:c829004-828735 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9673.26 Da
• Isoelectric Point: 6.8787

>AT233065 WP_236496341.1 NZ_CP091244:2345030-2345293 [Thiothrix winogradskyi]
MPMYDPAHPGEILGEDILKELNITITAAAEKLGISRKTLSAIVNGHAPITAETAIKLEKVFGKPDAEHWLRMQMTYDLYH
ARQKMAA

Nucleotide

• Length: 264 bp

>AT233065 NZ_CP128525:c829274-829008 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA