Detailed information of TA system TA023300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 537785..538464
• Replicon: chromosome
• Accession: NC_011740
• Organism: Escherichia fergusonii ATCC 35469

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: EFER_RS22700
• Protein ID: WP_002431667.1
• Coordinates: 537785..538087 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EFER_RS02830
• Protein ID: WP_000806441.1
• Coordinates: 538123..538464 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFER_RS02805	533161..534813	+	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
EFER_RS02810	534851..535354	-	504	WP_000667001.1	hypothetical protein	-
EFER_RS02815	535351..536151	-	801	WP_000439795.1	hypothetical protein	-
EFER_RS02820	536175..536654	-	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
EFER_RS02825	536858..537652	-	795	WP_000365145.1	TraB/GumN family protein	-
EFER_RS22700	537785..538087	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EFER_RS02830	538123..538464	+	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
EFER_RS02835	538578..541082	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
EFER_RS02840	541389..542678	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T23300 WP_002431667.1 NC_011740:537785-538087 [Escherichia fergusonii ATCC 35469]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T23300 NC_011740:537785-538087 [Escherichia fergusonii ATCC 35469]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT23300 WP_000806441.1 NC_011740:538123-538464 [Escherichia fergusonii ATCC 35469]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT23300 NC_011740:538123-538464 [Escherichia fergusonii ATCC 35469]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAGCCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACAACTG
AAATGGCGTTTCGTCTGGCTAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACACAAGAAGAATTGGGACGGATTGAAACAGTGGCTGACTATTTGGCTCGCCGTGAAGA
GCGTGCAAAGAATGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8