Detailed information of TA system TA023283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2280268..2280839
• Replicon: chromosome
• Accession: NC_011666
• Organism: Methylocella silvestris BL2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B8ERJ2
• Locus tag: MSIL_RS10750
• Protein ID: WP_012591114.1
• Coordinates: 2280268..2280549 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: MSIL_RS10755
• Protein ID: WP_012591115.1
• Coordinates: 2280558..2280839 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSIL_RS20185	2275325..2275978	-	654	WP_012591108.1	hypothetical protein	-
MSIL_RS10725	2276259..2276906	+	648	WP_012591109.1	HAD-IA family hydrolase	-
MSIL_RS10730	2276903..2277778	+	876	WP_012591110.1	ATPase	-
MSIL_RS10735	2277775..2278137	+	363	WP_012591111.1	hypothetical protein	-
MSIL_RS10740	2278201..2278698	+	498	WP_012591112.1	copper chaperone PCu(A)C	-
MSIL_RS10745	2278877..2279161	+	285	WP_012591113.1	hypothetical protein	-
MSIL_RS21320	2279711..2280115	-	405	WP_148213074.1	hypothetical protein	-
MSIL_RS10750	2280268..2280549	+	282	WP_012591114.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MSIL_RS10755	2280558..2280839	+	282	WP_012591115.1	HigA family addiction module antidote protein	Antitoxin
MSIL_RS10760	2280973..2281596	-	624	WP_012591116.1	riboflavin synthase	-
MSIL_RS10765	2281600..2282712	-	1113	WP_012591117.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
MSIL_RS10770	2282709..2283239	-	531	WP_012591118.1	transcriptional regulator NrdR	-
MSIL_RS10775	2283250..2284554	-	1305	WP_012591119.1	serine hydroxymethyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10687.22 Da
• Isoelectric Point: 8.7521

>T23283 WP_012591114.1 NC_011666:2280268-2280549 [Methylocella silvestris BL2]
MICSFAGRTTKAFFEDGKCPAKWSSFRAVALRKLDMLDAVAKLNDLASPPGNRLEALKHDRQGQYSIRINDQWRVCFRWT
ERGPADVEIVDYH

Nucleotide

• Length: 282 bp

>T23283 NC_011666:2280268-2280549 [Methylocella silvestris BL2]
ATGATTTGCAGCTTCGCCGGCCGGACGACGAAGGCCTTTTTCGAAGACGGCAAATGCCCGGCGAAATGGAGCTCGTTCCG
CGCGGTCGCCCTTCGCAAACTCGATATGCTTGATGCGGTCGCCAAGCTCAATGATTTGGCTTCGCCGCCAGGCAATCGCC
TCGAAGCCTTGAAGCACGATCGGCAGGGGCAATACAGCATCCGGATTAACGATCAATGGCGCGTCTGCTTTCGTTGGACG
GAGCGCGGCCCGGCAGACGTTGAAATTGTCGATTACCATTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10586.26 Da
• Isoelectric Point: 11.1911

>AT23283 WP_012591115.1 NC_011666:2280558-2280839 [Methylocella silvestris BL2]
MPRIRAHPGEILREEYLKPLGMSARGLAAALHVPPNRISEIVRERRDVTADTAIRLALCFRTTPQFWLNLQVAHDLSKAE
AGTDFRDVVRRRA

Nucleotide

• Length: 282 bp

>AT23283 NC_011666:2280558-2280839 [Methylocella silvestris BL2]
ATGCCCCGCATCCGCGCCCATCCCGGCGAAATTCTGCGCGAGGAATATCTCAAGCCGCTTGGGATGAGCGCGCGCGGCCT
GGCCGCTGCGCTTCACGTTCCCCCGAACCGGATTAGCGAGATTGTCCGCGAGCGCCGCGATGTGACCGCCGACACCGCAA
TCCGACTCGCGCTCTGTTTCAGGACCACGCCGCAGTTCTGGCTCAACCTGCAAGTCGCCCACGATCTCTCGAAGGCTGAA
GCCGGAACGGATTTCCGCGACGTCGTCCGGCGGCGCGCTTAA

Structures

Toxin

• AlphaFold DB: B8ERJ2