Detailed information of TA system TA232819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3851670..3852322
• Replicon: chromosome
• Accession: NZ_CP091125
• Organism: Serratia marcescens strain CM2015_854

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L2D47_RS18755
• Protein ID: WP_038878669.1
• Coordinates: 3851670..3852014 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GZD7
• Locus tag: L2D47_RS18760
• Protein ID: WP_038878665.1
• Coordinates: 3852020..3852322 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D47_RS18735 (L2D47_18730)	3846725..3846910	-	186	WP_038878678.1	hypothetical protein	-
L2D47_RS18740 (L2D47_18735)	3846911..3849169	-	2259	WP_038878674.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-
L2D47_RS18745 (L2D47_18740)	3849381..3850454	+	1074	WP_240285143.1	IS481-like element ISKpn28 family transposase	-
L2D47_RS18750 (L2D47_18745)	3850491..3851510	+	1020	WP_055312343.1	HTH-type transcriptional regulator GalR	-
L2D47_RS18755 (L2D47_18750)	3851670..3852014	+	345	WP_038878669.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2D47_RS18760 (L2D47_18755)	3852020..3852322	+	303	WP_038878665.1	XRE family transcriptional regulator	Antitoxin
L2D47_RS18765 (L2D47_18760)	3852350..3853612	-	1263	WP_038878662.1	diaminopimelate decarboxylase	-
L2D47_RS18770 (L2D47_18765)	3853746..3854669	+	924	WP_080491086.1	LysR family transcriptional regulator	-
L2D47_RS18775 (L2D47_18770)	3854697..3855605	-	909	WP_015378928.1	LysR family transcriptional regulator	-
L2D47_RS18780 (L2D47_18775)	3855714..3856598	+	885	WP_041036524.1	MBL fold metallo-hydrolase	-
L2D47_RS18785 (L2D47_18780)	3856667..3857314	+	648	WP_047729966.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3849381..3850454	1073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13367.40 Da
• Isoelectric Point: 10.6629

>T232819 WP_038878669.1 NZ_CP091125:3851670-3852014 [Serratia marcescens]
MWQVVTVERFDDWFLALNNAQQTSILAAIFKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDQRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T232819 NZ_CP128256:c85286-84906 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11111.04 Da
• Isoelectric Point: 10.3986

>AT232819 WP_038878665.1 NZ_CP091125:3852020-3852322 [Serratia marcescens]
MAKPLSQLIDKLDPAVVATARQKADKEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT232819 NZ_CP128256:c85507-85286 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGAAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GZD7