Detailed information of TA system TA232800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4220903..4221555
• Replicon: chromosome
• Accession: NZ_CP091123
• Organism: Serratia marcescens strain CM2017_569

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L2D48_RS20125
• Protein ID: WP_038878669.1
• Coordinates: 4220903..4221247 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GZD7
• Locus tag: L2D48_RS20130
• Protein ID: WP_038878665.1
• Coordinates: 4221253..4221555 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D48_RS20115 (L2D48_20115)	4217245..4219503	-	2259	WP_240296036.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-
L2D48_RS20120 (L2D48_20120)	4219724..4220743	+	1020	WP_038878671.1	HTH-type transcriptional regulator GalR	-
L2D48_RS20125 (L2D48_20125)	4220903..4221247	+	345	WP_038878669.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2D48_RS20130 (L2D48_20130)	4221253..4221555	+	303	WP_038878665.1	XRE family transcriptional regulator	Antitoxin
L2D48_RS20135 (L2D48_20135)	4221583..4222845	-	1263	WP_038878662.1	diaminopimelate decarboxylase	-
L2D48_RS20140 (L2D48_20140)	4222979..4223902	+	924	WP_038878659.1	LysR family transcriptional regulator	-
L2D48_RS20145 (L2D48_20145)	4223930..4224838	-	909	WP_038878655.1	LysR family transcriptional regulator	-
L2D48_RS20150 (L2D48_20150)	4224947..4225831	+	885	WP_033635778.1	MBL fold metallo-hydrolase	-
L2D48_RS20155 (L2D48_20155)	4225900..4226547	+	648	WP_038878652.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13367.40 Da
• Isoelectric Point: 10.6629

>T232800 WP_038878669.1 NZ_CP091123:4220903-4221247 [Serratia marcescens]
MWQVVTVERFDDWFLALNNAQQTSILAAIFKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDQRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T232800 NZ_CP128233:2763845-2764312 [Paralcaligenes sp. CC-YST667]
ATGAGCGCAAGGAAGCCTGAGCCCTTGGTCATTCATGGCTGGACGGTCTTTGCCCATCCGTTGTTCCTCGCACAGATTGA
AGCGCTGGTGCGGCAGGTCGAGAGCCTGAAGCAGAAGGACCCGGCCGGGTACGTAAAAAAGAACGCCAGCAAGCGGCTGG
CTGCTATCACCAAACTGGCGTTCGATGTCATCCCGCAAGATCCGACGCGACCGGAGTACCGCCAGGGTGGGGCGCTCGGT
GACGATCACAAGCACTGGTTCCGGGCCAAGTTCTTCCAGCAGTACCGCCTATTCTTCCGCTACCACGCCCCGAGCAAAGT
GATTGTCTACGCCTGGGTGAGTGACGAAGACACCAAGCGCGCCTACGAAAGCAGCGACGACGCCTACCGGGTGTTCCGCA
AGATGCTGGAAAGCGGGCATCCGCCTGATGACTGGGATCAGCTCCTGGCTGAGGCCGTAACCAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11111.04 Da
• Isoelectric Point: 10.3986

>AT232800 WP_038878665.1 NZ_CP091123:4221253-4221555 [Serratia marcescens]
MAKPLSQLIDKLDPAVVATARQKADKEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT232800 NZ_CP128233:2763519-2763848 [Paralcaligenes sp. CC-YST667]
ATGGCCGCAACCCTCGAAGTCGAATCCACACTCACCGATCGCTACCAGACCACGGTGCCGGAAACCGTGCGTCGTGCCCT
CAAGCTGGGCAAGCGCGACAAGATCCACTACACCATTCACCCCAGCGGCGATGTGGTGCTGACGCGCGTCGAAGCATCCG
ACGGTGATGACCCGGTGCTTGGTCAGTTCCTCGCGTTCCTGGCCGCCGACATCACTCGTCATCCCGAGCGGCTGCAAGCT
GTGGATGCCGGCCTCGCCCAGCGGCTCCAGGCGCTGGTCGGCGGCGTCGATGTCGATCTCAATGCCGCCCTGTCGGCAGA
CGATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GZD7