Detailed information of TA system TA232766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4582614..4583344
• Replicon: chromosome
• Accession: NZ_CP091121
• Organism: Serratia ureilytica strain CM2016_324

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: L2D49_RS21860
• Protein ID: WP_004937033.1
• Coordinates: 4582614..4582925 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2D49_RS21865
• Protein ID: WP_089192287.1
• Coordinates: 4582922..4583344 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2D49_RS21830 (L2D49_21830)	4577929..4578654	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
L2D49_RS21835 (L2D49_21835)	4578707..4580140	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
L2D49_RS21840 (L2D49_21840)	4580164..4580451	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
L2D49_RS21845 (L2D49_21845)	4580607..4581068	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
L2D49_RS21850 (L2D49_21850)	4581196..4582050	+	855	WP_015379177.1	RNase adapter RapZ	-
L2D49_RS21855 (L2D49_21855)	4582047..4582319	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
L2D49_RS21860 (L2D49_21860)	4582614..4582925	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
L2D49_RS21865 (L2D49_21865)	4582922..4583344	+	423	WP_089192287.1	helix-turn-helix domain-containing protein	Antitoxin
L2D49_RS21870 (L2D49_21870)	4583351..4585267	-	1917	WP_033644486.1	PRD domain-containing protein	-
L2D49_RS21875 (L2D49_21875)	4585319..4586533	-	1215	WP_072626914.1	lactonase family protein	-
L2D49_RS21880 (L2D49_21880)	4586621..4587361	-	741	WP_187048420.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T232766 WP_004937033.1 NZ_CP091121:4582614-4582925 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T232766 NZ_CP128218:2396949-2397278 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15752.07 Da
• Isoelectric Point: 5.2060

>AT232766 WP_089192287.1 NZ_CP091121:4582922-4583344 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYRDALELVEYLLENDERHPLIDLLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT232766 NZ_CP128218:2396611-2396928 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63