Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4519358..4519986 Replicon chromosome
Accession NZ_CP091121
Organism Serratia ureilytica strain CM2016_324

Toxin (Protein)


Gene name higB Uniprot ID A0A8B4GSN3
Locus tag L2D49_RS21540 Protein ID WP_042785415.1
Coordinates 4519358..4519648 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag L2D49_RS21545 Protein ID WP_015379150.1
Coordinates 4519663..4519986 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2D49_RS21525 (L2D49_21525) 4514468..4516489 + 2022 WP_122004384.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L2D49_RS21530 (L2D49_21530) 4516630..4517847 - 1218 Protein_4197 IS3 family transposase -
L2D49_RS21535 (L2D49_21535) 4517988..4519127 - 1140 WP_033644533.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L2D49_RS21540 (L2D49_21540) 4519358..4519648 + 291 WP_042785415.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L2D49_RS21545 (L2D49_21545) 4519663..4519986 + 324 WP_015379150.1 HigA family addiction module antitoxin Antitoxin
L2D49_RS21550 (L2D49_21550) 4519979..4520491 + 513 WP_042785417.1 M48 family metallopeptidase -
L2D49_RS21555 (L2D49_21555) 4520542..4521522 + 981 WP_072626939.1 Gfo/Idh/MocA family oxidoreductase -
L2D49_RS21560 (L2D49_21560) 4521524..4522255 + 732 WP_033644531.1 glutamine amidotransferase -
L2D49_RS21565 (L2D49_21565) 4522508..4523476 + 969 WP_016930179.1 TerC family protein -
L2D49_RS21570 (L2D49_21570) 4523740..4524975 + 1236 WP_028128305.1 serine/threonine transporter SstT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4516630..4517433 803


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11672.37 Da        Isoelectric Point: 10.4297

>T232765 WP_042785415.1 NZ_CP091121:4519358-4519648 [Serratia ureilytica]
MIKSFRDRYLEQFYLEGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPKGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

>T232765 NZ_CP128218:2385414-2386487 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT232765 WP_015379150.1 NZ_CP091121:4519663-4519986 [Serratia ureilytica]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT232765 NZ_CP128218:2386480-2386851 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4GSN3

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References