Detailed information of TA system TA023274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4256836..4257448
• Replicon: chromosome
• Accession: NC_011662
• Organism: Thauera sp. MZ1T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: TMZ1T_RS18710
• Protein ID: WP_012586183.1
• Coordinates: 4257110..4257448 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C4KD37
• Locus tag: TMZ1T_RS18705
• Protein ID: WP_012586182.1
• Coordinates: 4256836..4257117 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMZ1T_RS18675	4253243..4253968	+	726	WP_004300250.1	YebC/PmpR family DNA-binding transcriptional regulator	-
TMZ1T_RS18680	4254036..4254917	+	882	WP_004300252.1	DMT family transporter	-
TMZ1T_RS18685	4254952..4255269	+	318	WP_004300255.1	heavy metal-binding domain-containing protein	-
TMZ1T_RS18690	4255266..4255817	+	552	WP_004300257.1	crossover junction endodeoxyribonuclease RuvC	-
TMZ1T_RS20725	4256497..4256814	-	318	WP_012586181.1	HigA family addiction module antidote protein	-
TMZ1T_RS18705	4256836..4257117	-	282	WP_012586182.1	helix-turn-helix domain-containing protein	Antitoxin
TMZ1T_RS18710	4257110..4257448	-	339	WP_012586183.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMZ1T_RS18715	4257868..4258449	-	582	WP_004300281.1	phasin family protein	-
TMZ1T_RS18720	4258599..4258817	+	219	WP_012586184.1	hypothetical protein	-
TMZ1T_RS18725	4258896..4260218	-	1323	WP_012586185.1	IS1182 family transposase	-
TMZ1T_RS18730	4260299..4260838	+	540	Protein_3748	hypothetical protein	-
TMZ1T_RS18735	4261459..4262106	+	648	WP_004300285.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4258896..4260218	1322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-82)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12781.98 Da
• Isoelectric Point: 10.8258

>T23274 WP_012586183.1 NC_011662:c4257448-4257110 [Thauera sp. MZ1T]
MRYSMHWTVTVIPEAEAELLALPADMQARFLHIAELLESFGPQKVGMPHIRPLEGKLWEMRMTGRDGIARAVYVARTGQR
LTVLHVFTKKTQKTPRKAIETAQARLRSLTND

Nucleotide

• Length: 339 bp

>T23274 NC_011662:c4257448-4257110 [Thauera sp. MZ1T]
ATGCGATACTCTATGCACTGGACCGTTACCGTTATCCCGGAGGCCGAGGCCGAATTGCTGGCGCTGCCCGCCGACATGCA
AGCCCGCTTCCTGCACATTGCCGAGTTGCTGGAATCCTTCGGCCCGCAGAAGGTGGGCATGCCGCATATTCGGCCGCTGG
AAGGGAAACTGTGGGAAATGCGCATGACCGGCCGCGACGGCATCGCCCGCGCCGTCTATGTGGCCCGCACCGGGCAGCGC
CTGACGGTCCTGCACGTCTTCACCAAGAAGACCCAGAAAACTCCGCGCAAGGCCATCGAAACGGCCCAAGCGCGACTGAG
GAGCCTGACCAATGACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10290.70 Da
• Isoelectric Point: 9.9599

>AT23274 WP_012586182.1 NC_011662:c4257117-4256836 [Thauera sp. MZ1T]
MTELAALKARILANPEARAEYDAQAPEFELARELITARTRAGLTQSELAERMHTTQSTIARLESGRTMPSMRTLARYAEA
TGSRAVVRLERSA

Nucleotide

• Length: 282 bp

>AT23274 NC_011662:c4257117-4256836 [Thauera sp. MZ1T]
ATGACTGAGCTTGCCGCCCTGAAGGCCCGCATTCTGGCGAACCCCGAGGCCCGCGCCGAGTACGACGCGCAAGCGCCGGA
GTTCGAGCTTGCCCGCGAACTCATCACCGCCCGCACCCGCGCCGGCCTGACTCAATCCGAGCTTGCCGAGCGCATGCACA
CGACGCAGTCGACCATCGCCCGCCTGGAGAGTGGCCGCACCATGCCGAGCATGCGCACGCTGGCCCGCTATGCCGAGGCG
ACCGGAAGCCGCGCGGTGGTGCGCCTGGAGCGCAGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C3HX94