Detailed information of TA system TA232721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 646432..647762
• Replicon: chromosome
• Accession: NZ_CP091099
• Organism: Pantoea agglomerans strain Pa58

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: LZ609_RS06540
• Protein ID: WP_245116378.1
• Coordinates: 646432..647454 (-)
• Length: 341 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A7X2SVD5
• Locus tag: LZ609_RS06545
• Protein ID: WP_010670935.1
• Coordinates: 647451..647762 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ609_RS06535 (LZ609_06535)	644087..646306	+	2220	WP_222939431.1	catalase/peroxidase HPI	-
LZ609_RS06540 (LZ609_06540)	646432..647454	-	1023	WP_245116378.1	HipA domain-containing protein	Toxin
LZ609_RS06545 (LZ609_06545)	647451..647762	-	312	WP_010670935.1	HipA N-terminal domain-containing protein	Antitoxin
LZ609_RS06550 (LZ609_06550)	647747..648082	-	336	WP_050680122.1	XRE family transcriptional regulator	-
LZ609_RS06560 (LZ609_06560)	648861..649982	+	1122	WP_245116380.1	Gfo/Idh/MocA family oxidoreductase	-
LZ609_RS06565 (LZ609_06565)	650073..650903	-	831	WP_245116382.1	glycosyltransferase family 8 protein	-
LZ609_RS06570 (LZ609_06570)	650916..652298	-	1383	WP_245116384.1	D-arabinono-1,4-lactone oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (58-302)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 341 a.a.
• Molecular weight: 38480.01 Da
• Isoelectric Point: 6.6643

>T232721 WP_245116378.1 NZ_CP091099:c647454-646432 [Pantoea agglomerans]
MNSERCLITLKRLKESELKTGYSTKGIRHLLGSLNVALSLPFTRSQFIQELPERQKGMSISGYQPKLSLRLTKGELVVVS
NDGTYILKPSPEEYPHLAENEHATMCVMQRLKFDVPPFGLMPFSHDENRPDELAFVIKRYDRTASDNARLHQEQLDGAMG
LGDKYGRIDNHKAISYEKAAKFLNTRVNSSLKSKRDLFLRIVYAYVLGNNDFHLRNIGIILPPQGSPWLAPVYDFISVVP
YPAAFGEYLALPLLEREENDAGNAPGINSEYGEYLGADFILLAQGMDINPTLARKWLSEVVSHHQLIIDTYNESHMPESQ
REAVLDYVARRITLLAVTEI

Nucleotide

• Length: 1023 bp

>T232721 NZ_CP128188:228520-228786 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11668.44 Da
• Isoelectric Point: 4.7953

>AT232721 WP_010670935.1 NZ_CP091099:c647762-647451 [Pantoea agglomerans]
MARKIEVWLYNTLAGTLSEGQNGYTFCYLPTYAGCAISLSMPVRPEPYLSEELHPFFKGLAPEGWLRKRYSEIQKIDDKD
LFGLLMENGNDLLGAVVFKEITS

Nucleotide

• Length: 312 bp

>AT232721 NZ_CP128188:228257-228520 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X2SVD5