Detailed information of TA system TA232717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 149681..150502
• Replicon: plasmid p1
• Accession: NZ_CP091097
• Organism: Pantoea agglomerans strain Pa58

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: LZ609_RS00760
• Protein ID: WP_010670281.1
• Coordinates: 150032..150502 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: LZ609_RS00755
• Protein ID: WP_089414818.1
• Coordinates: 149681..150025 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ609_RS00735 (LZ609_00735)	145682..146791	+	1110	WP_010247360.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
LZ609_RS00740 (LZ609_00740)	146835..148136	+	1302	WP_033760237.1	maltoporin	-
LZ609_RS00745 (LZ609_00745)	148239..149177	+	939	WP_163641413.1	maltose operon protein MalM	-
LZ609_RS00750 (LZ609_00750)	149366..149581	+	216	WP_010670279.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LZ609_RS00755 (LZ609_00755)	149681..150025	+	345	WP_089414818.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
LZ609_RS00760 (LZ609_00760)	150032..150502	+	471	WP_010670281.1	type II toxin-antitoxin system YhaV family toxin	Toxin
LZ609_RS00765 (LZ609_00765)	150690..152765	-	2076	WP_245115216.1	4-alpha-glucanotransferase	-
LZ609_RS00770 (LZ609_00770)	152774..155176	-	2403	WP_245115217.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..554101	554101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (13-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18238.78 Da
• Isoelectric Point: 8.9262

>T232717 WP_010670281.1 NZ_CP091097:150032-150502 [Pantoea agglomerans]
MEDSTVINGWHVYVPPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQDWANTNS

Nucleotide

• Length: 471 bp

>T232717 NZ_CP128182:266417-266719 [Stenotrophomonas maltophilia]
ATGGCTATCAAGAAGAAAGTTGAACTCGAAGCATTTGATGTGGCCGAACACCTTCATACACCGAAGGAAATGGCGGCCTA
TCTCGATGCATGCATCGAAGAAAGCGACGGCGATTCGGCATTCATCGCCAAGGCGCTGGGCGATATCGCGCGTGCCCAAG
GGATGAGCAAGGTCGCACGTGCGGCGGGCCTGTCGCGGGAGAGTCTGTATCGTGCCCTGTCTGGCGAGCGCAGCCCTGAT
TTCTCGACCATCCTGAAGGTGACTCGTGCCCTGGGTGTGCGCCTGCATGCCAGCGCTGCATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12252.00 Da
• Isoelectric Point: 4.7969

>AT232717 WP_089414818.1 NZ_CP091097:149681-150025 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARGQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVADDAPVKASFLSFLPADRRNT
AANIKMLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT232717 NZ_CP128182:266071-266376 [Stenotrophomonas maltophilia]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCTGGTGATCACCGCTATCTGGCGGATGGCGTTTCTGAATTGCGTATCGATG
CAGGACCCGGTTACCGCGTGTACTACACCCAGCGGGGCAGGCAGTTGGTGATTCTTCTGGTCGGAGGCGACAAGGGTTCG
CAACAGCGCGATATAGAGAAGGCCAAGGAGATTGCGCGTGCCCTGCGAGCATCGAACTGGTTGTAG