Detailed information of TA system TA232714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1146708..1147294
• Replicon: chromosome
• Accession: NZ_CP091088
• Organism: Pseudomonas juntendi strain PP_2463

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A6I2K6L8
• Locus tag: L1P09_RS05215
• Protein ID: WP_029885077.1
• Coordinates: 1147016..1147294 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: L1P09_RS05210
• Protein ID: WP_029885078.1
• Coordinates: 1146708..1147007 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P09_RS05185 (L1P09_05185)	1142594..1143403	+	810	WP_009686964.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
L1P09_RS05190 (L1P09_05190)	1143475..1143885	-	411	WP_054881203.1	SufE family protein	-
L1P09_RS05195 (L1P09_05195)	1143882..1145087	-	1206	WP_029885081.1	cysteine desulfurase	-
L1P09_RS05200 (L1P09_05200)	1145169..1146203	-	1035	WP_009686960.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
L1P09_RS05205 (L1P09_05205)	1146235..1146582	-	348	WP_054881204.1	ArsC family reductase	-
L1P09_RS05210 (L1P09_05210)	1146708..1147007	-	300	WP_029885078.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
L1P09_RS05215 (L1P09_05215)	1147016..1147294	-	279	WP_029885077.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1P09_RS05220 (L1P09_05220)	1147543..1149189	+	1647	WP_029885076.1	Na+/H+ antiporter	-
L1P09_RS05225 (L1P09_05225)	1149734..1150930	-	1197	WP_029888073.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.72 Da
• Isoelectric Point: 7.1344

>T232714 WP_029885077.1 NZ_CP091088:c1147294-1147016 [Pseudomonas juntendi]
MIQSFSCADTEALFVTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLEALSGNRAGQHSIRVNDQWRLCFTWTD
NGPANVEIIDYH

Nucleotide

• Length: 279 bp

>T232714 NZ_CP128169:c1610668-1610405 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACACCTTGGCACCCAACCAAGGCTTTGAGAAATTAGTACCGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGTACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATTACGAATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10903.58 Da
• Isoelectric Point: 6.4603

>AT232714 WP_029885078.1 NZ_CP091088:c1147007-1146708 [Pseudomonas juntendi]
MLKNGMRPIHPGEVLREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQRHGEEIIGAVQRLVA

Nucleotide

• Length: 300 bp

>AT232714 NZ_CP128169:c1610919-1610665 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAAGGCGGATGAAAAGAGCGTTGTCGTCATTAGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Structures

Toxin

• AlphaFold DB: A0A6I2K6L8