Detailed information of TA system TA232687

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102034..102287
• Replicon: plasmid pWSCRKP-2
• Accession: NZ_CP091070
• Organism: Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L0980_RS28840
• Protein ID: WP_001312851.1
• Coordinates: 102138..102287 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	102034..102093 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0980_RS28800 (97416)	97416..97760	-	345	Protein_139	IS6-like element IS26 family transposase	-
L0980_RS28805 (97812)	97812..98516	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
L0980_RS28810 (98541)	98541..98741	+	201	WP_072354025.1	hypothetical protein	-
L0980_RS28815 (98761)	98761..99507	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
L0980_RS28820 (99562)	99562..100122	+	561	WP_000139328.1	fertility inhibition protein FinO	-
L0980_RS28825 (100254)	100254..100454	+	201	WP_015059022.1	hypothetical protein	-
L0980_RS28830 (100840)	100840..101439	+	600	WP_032083981.1	PIN domain-containing protein	-
L0980_RS28835 (101501)	101501..101833	+	333	WP_152916585.1	hypothetical protein	-
- (102034)	102034..102093	-	60	NuclAT_1	-	Antitoxin
- (102034)	102034..102093	-	60	NuclAT_1	-	Antitoxin
- (102034)	102034..102093	-	60	NuclAT_1	-	Antitoxin
- (102034)	102034..102093	-	60	NuclAT_1	-	Antitoxin
L0980_RS28840 (102138)	102138..102287	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
L0980_RS28845 (102571)	102571..102819	+	249	WP_000083837.1	replication regulatory protein RepA	-
L0980_RS28850 (102934)	102934..103118	+	185	Protein_149	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2	-	1..103130	103130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T232687 WP_001312851.1 NZ_CP091070:102138-102287 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T232687 NZ_CP127881:c3667642-3667286 [Serratia marcescens]
ATGATCTTTTTGACCGCAGAGGATATTGCGGAATTCAATGCTGAAATTGTGCCTCACGGCCGCCAGGATGGCAGCAAAGT
TGAGGCTGTAGCCAACCGCGAGCTCAATGCTTACCACTATGAGAACGTCACCGACGTTTACCGTTTGGCCGCACTTTATC
TCATTGCTATCAGTCATGGTCATATTTTTCTTGATGACAATAAGCGCACGGCCTTCCAAAGCATGGCGTTATTTCTCGGT
ATCAATGGCATTGCACTGCGTGAAGACGCAGAACTGGTCGAAATTACGGTTGAAGCAGCTCAAGGCCGCCTCAACGTTGA
ACAAGCCGCGGAGAAACTGCGCCGGCTAACCGAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT232687 NZ_CP091070:c102093-102034 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin