Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 61267..61994 | Replicon | plasmid pWSCRKP-1 |
| Accession | NZ_CP091069 | ||
| Organism | Klebsiella pneumoniae strain WSCRKP | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | L0980_RS27205 | Protein ID | WP_011251285.1 |
| Coordinates | 61683..61994 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | L0980_RS27200 | Protein ID | WP_011251286.1 |
| Coordinates | 61267..61686 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L0980_RS27170 (L0980_27170) | 56435..56905 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| L0980_RS27175 (L0980_27175) | 57218..57853 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| L0980_RS27180 (L0980_27180) | 58272..58892 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| L0980_RS27185 (L0980_27185) | 58913..59701 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| L0980_RS27190 (L0980_27190) | 59715..60080 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| L0980_RS27195 (L0980_27195) | 60152..61120 | - | 969 | WP_086642568.1 | IS5 family transposase | - |
| L0980_RS27200 (L0980_27200) | 61267..61686 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| L0980_RS27205 (L0980_27205) | 61683..61994 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| L0980_RS27210 (L0980_27210) | 62199..62636 | - | 438 | Protein_62 | DDE-type integrase/transposase/recombinase | - |
| L0980_RS27215 (L0980_27215) | 62771..63468 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| L0980_RS27220 (L0980_27220) | 63470..63919 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| L0980_RS27225 (L0980_27225) | 63936..64247 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| L0980_RS27230 (L0980_27230) | 64261..64602 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| L0980_RS27235 (L0980_27235) | 64662..65618 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| L0980_RS27240 (L0980_27240) | 66043..66483 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| L0980_RS27245 (L0980_27245) | 66489..66983 | - | 495 | WP_011251274.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..221776 | 221776 | |
| - | inside | IScluster/Tn | - | - | 44596..68903 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T232681 WP_011251285.1 NZ_CP091069:c61994-61683 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T232681 NZ_CP127881:c19931-19551 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATTCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTTT
GACCGAATACCACACCAATCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAGCAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATTCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTTT
GACCGAATACCACACCAATCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAGCAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT232681 WP_011251286.1 NZ_CP091069:c61686-61267 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT232681 NZ_CP127881:c20152-19931 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGCTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGCTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|