Detailed information of TA system TA023266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4389128..4389730
• Replicon: chromosome
• Accession: NC_011601
• Organism: Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: E2348C_RS22515
• Protein ID: WP_000897305.1
• Coordinates: 4389128..4389439 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2348C_RS22520
• Protein ID: WP_000356397.1
• Coordinates: 4389440..4389730 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2348C_RS22490 (E2348_C_4186)	4385042..4385641	+	600	WP_001298594.1	glucose-1-phosphatase	-
E2348C_RS22495 (E2348_C_4187)	4385635..4386507	+	873	WP_000920747.1	virulence factor BrkB family protein	-
E2348C_RS22500 (E2348_C_4188)	4386504..4386941	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
E2348C_RS22505 (E2348_C_4189)	4386986..4387927	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
E2348C_RS22510 (E2348_C_4190)	4387991..4388899	-	909	WP_001386513.1	alpha/beta hydrolase	-
E2348C_RS22515 (E2348_C_4191)	4389128..4389439	+	312	WP_000897305.1	hypothetical protein	Toxin
E2348C_RS22520 (E2348_C_4192)	4389440..4389730	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
E2348C_RS22525 (E2348_C_4193)	4390335..4390553	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
E2348C_RS22530 (E2348_C_4194)	4390777..4391706	-	930	WP_000027712.1	formate dehydrogenase accessory protein FdhE	-
E2348C_RS22535 (E2348_C_4195)	4391703..4392338	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
E2348C_RS22540 (E2348_C_4196)	4392335..4393237	-	903	WP_000331385.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T23266 WP_000897305.1 NC_011601:4389128-4389439 [Escherichia coli O127:H6 str. E2348/69]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T23266 NC_011601:4389128-4389439 [Escherichia coli O127:H6 str. E2348/69]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT23266 WP_000356397.1 NC_011601:4389440-4389730 [Escherichia coli O127:H6 str. E2348/69]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT23266 NC_011601:4389440-4389730 [Escherichia coli O127:H6 str. E2348/69]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7