Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 242448..242989 Replicon chromosome
Accession NZ_CP091065
Organism Escherichia coli strain DOC14

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag L1123_RS01135 Protein ID WP_000615983.1
Coordinates 242448..242726 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag L1123_RS01140 Protein ID WP_000729703.1
Coordinates 242729..242989 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1123_RS01120 (240030) 240030..240608 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
L1123_RS01125 (240814) 240814..241581 + 768 WP_000333380.1 class II glutamine amidotransferase -
L1123_RS01130 (241552) 241552..242292 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L1123_RS01135 (242448) 242448..242726 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L1123_RS01140 (242729) 242729..242989 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L1123_RS01145 (243199) 243199..243948 + 750 WP_000056849.1 C40 family peptidase -
L1123_RS01150 (244124) 244124..244621 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L1123_RS01155 (244845) 244845..246557 - 1713 Protein_223 flagellar biosynthesis protein FlhA -
L1123_RS01160 (246529) 246529..247314 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T232609 WP_000615983.1 NZ_CP091065:c242726-242448 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T232609 NZ_CP127409:3124897-3125178 [Rhodanobacter thiooxydans]
ATGATATGGAGCTTCGCGGACAAGGAGGCGGAGAAGATCTGGGGTGGTACGCCATCTCGGCGTTTGCCCATGGACATCCA
GCCGGTCGCGCGCCGCAAATTGCGCATGCTCAACAACGCCTCGACGCTGGACGATTTGTGTGTTCCTCCGGCCAACCGCC
TCGAAGCGTTGAAGGGTGACCGCAAGGGCTGGCACAGCATCCGCATCAACGACCAATGGCGCATCTGCTTCCACTGGAAG
GACGGCGATGCCCTCGACGTGGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT232609 WP_000729703.1 NZ_CP091065:c242989-242729 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT232609 NZ_CP127409:3125194-3125484 [Rhodanobacter thiooxydans]
ATGAAGACCCTGCCCAACATCCATCCCGGCGAAGTGCTCCTCGAAGAGTTCCTGCTGCCGCTGAACATCAGCCAGAATGC
CCTCGCTCGCGCCGCTGGCGTCCCGCCGCGCCGCATCAACGAAATCGTGCTCGGCAAGCGTGGCGTCACCGCCGATACTG
CAGTGCGGCTCGCGGCCGCACTCGGCACCAGCGAGCGCTTCTGGTTGGGTCTGCAGGCCGACTATGACCTGGAGGAAGCG
CATCGGGCGCTGGGCAGGGCGGTGAAACGCATCGAGCGGATCGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References