23260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2207660..2208162 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E2348C_RS11570 Protein ID WP_000767829.1
Coordinates 2207660..2207914 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E2348C_RS11575 Protein ID WP_001259255.1
Coordinates 2207911..2208162 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS11555 (E2348_C_2154) 2204153..2205511 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
E2348C_RS28450 2205501..2205563 - 63 WP_010723108.1 membrane protein YoeI -
E2348C_RS11560 (E2348_C_2155) 2205778..2206707 - 930 WP_000803379.1 LysR family transcriptional regulator -
E2348C_RS11565 (E2348_C_2156) 2206753..2207577 - 825 WP_000754737.1 SDR family oxidoreductase -
E2348C_RS11570 (E2348_C_2157) 2207660..2207914 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E2348C_RS11575 (E2348_C_2158) 2207911..2208162 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E2348C_RS27140 (E2348C_2159) 2208445..2208495 + 51 WP_001364200.1 his operon leader peptide -
E2348C_RS11580 (E2348_C_2160) 2208641..2209540 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E2348C_RS11585 (E2348_C_2161) 2209546..2210850 + 1305 WP_001339747.1 histidinol dehydrogenase -
E2348C_RS11590 (E2348_C_2162) 2210847..2211917 + 1071 WP_000108994.1 histidinol-phosphate transaminase -
E2348C_RS11595 (E2348_C_2163) 2211917..2212984 + 1068 WP_000080126.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T23260 WP_000767829.1 NC_011601:c2207914-2207660 [Escherichia coli O127:H6 str. E2348/69]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T23260 NC_011601:c2207914-2207660 [Escherichia coli O127:H6 str. E2348/69]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT23260 WP_001259255.1 NC_011601:c2208162-2207911 [Escherichia coli O127:H6 str. E2348/69]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT23260 NC_011601:c2208162-2207911 [Escherichia coli O127:H6 str. E2348/69]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References