Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2207660..2208162 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E2348C_RS11570 Protein ID WP_000767829.1
Coordinates 2207660..2207914 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E2348C_RS11575 Protein ID WP_001259255.1
Coordinates 2207911..2208162 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS11555 (E2348_C_2154) 2204153..2205511 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
E2348C_RS28450 2205501..2205563 - 63 WP_010723108.1 membrane protein YoeI -
E2348C_RS11560 (E2348_C_2155) 2205778..2206707 - 930 WP_000803379.1 LysR family transcriptional regulator -
E2348C_RS11565 (E2348_C_2156) 2206753..2207577 - 825 WP_000754737.1 SDR family oxidoreductase -
E2348C_RS11570 (E2348_C_2157) 2207660..2207914 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E2348C_RS11575 (E2348_C_2158) 2207911..2208162 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E2348C_RS27140 (E2348C_2159) 2208445..2208495 + 51 WP_001364200.1 his operon leader peptide -
E2348C_RS11580 (E2348_C_2160) 2208641..2209540 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E2348C_RS11585 (E2348_C_2161) 2209546..2210850 + 1305 WP_001339747.1 histidinol dehydrogenase -
E2348C_RS11590 (E2348_C_2162) 2210847..2211917 + 1071 WP_000108994.1 histidinol-phosphate transaminase -
E2348C_RS11595 (E2348_C_2163) 2211917..2212984 + 1068 WP_000080126.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T23260 WP_000767829.1 NC_011601:c2207914-2207660 [Escherichia coli O127:H6 str. E2348/69]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T23260 NC_011601:c2207914-2207660 [Escherichia coli O127:H6 str. E2348/69]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT23260 WP_001259255.1 NC_011601:c2208162-2207911 [Escherichia coli O127:H6 str. E2348/69]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT23260 NC_011601:c2208162-2207911 [Escherichia coli O127:H6 str. E2348/69]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References