Detailed information of TA system TA232546

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66611..66865
• Replicon: plasmid p3_STEC346
• Accession: NZ_CP091047
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L1P11_RS26685
• Protein ID: WP_001312851.1
• Coordinates: 66716..66865 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66611..66669 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS26660 (63101)	63101..63322	+	222	WP_000412083.1	hypothetical protein	-
L1P11_RS26665 (63330)	63330..64079	+	750	WP_000123038.1	type-F conjugative transfer system pilin acetylase TraX	-
L1P11_RS26670 (64153)	64153..64713	+	561	WP_000139377.1	fertility inhibition protein FinO	-
L1P11_RS26675 (64846)	64846..65046	+	201	WP_032288239.1	hypothetical protein	-
L1P11_RS26680 (65425)	65425..66024	+	600	WP_000256073.1	PIN domain-containing protein	-
- (66611)	66611..66669	-	59	NuclAT_1	-	Antitoxin
- (66611)	66611..66669	-	59	NuclAT_1	-	Antitoxin
- (66611)	66611..66669	-	59	NuclAT_1	-	Antitoxin
- (66611)	66611..66669	-	59	NuclAT_1	-	Antitoxin
L1P11_RS26685 (66716)	66716..66865	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
L1P11_RS26690 (67133)	67133..67387	+	255	WP_000083830.1	replication regulatory protein RepA	-
L1P11_RS26695 (67625)	67625..67699	+	75	WP_001365705.1	RepA leader peptide Tap	-
L1P11_RS26700 (67692)	67692..68549	+	858	WP_000130986.1	incFII family plasmid replication initiator RepA	-
L1P11_RS26705 (69249)	69249..69389	+	141	WP_161710125.1	hypothetical protein	-
L1P11_RS26710 (69548)	69548..69877	+	330	WP_000493287.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L1P11_RS26715 (69858)	69858..70139	+	282	WP_000780220.1	helix-turn-helix transcriptional regulator	-
L1P11_RS26720 (70207)	70207..71454	-	1248	Protein_91	Tn3-like element Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A)	-	1..82263	82263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T232546 WP_001312851.1 NZ_CP091047:66716-66865 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T232546 NZ_CP127316:4591695-4592093 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT232546 NZ_CP091047:c66669-66611 [Escherichia coli]
AGATACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin