Detailed information of TA system TA232542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26239..26508
• Replicon: plasmid p3_STEC346
• Accession: NZ_CP091047
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L1P11_RS26460
• Protein ID: WP_001372321.1
• Coordinates: 26383..26508 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26239..26304 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS26425	21291..21527	+	237	WP_071531732.1	hypothetical protein	-
L1P11_RS26430	21999..22532	+	534	WP_000290815.1	single-stranded DNA-binding protein	-
L1P11_RS26435	22589..22822	+	234	WP_001519997.1	DUF905 family protein	-
L1P11_RS26440	22887..24851	+	1965	WP_235412082.1	ParB/RepB/Spo0J family partition protein	-
L1P11_RS26445	24920..25354	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-
L1P11_RS26450	25351..26113	+	763	Protein_37	plasmid SOS inhibition protein A	-
-	26082..26306	+	225	NuclAT_0	-	-
-	26082..26306	+	225	NuclAT_0	-	-
-	26082..26306	+	225	NuclAT_0	-	-
-	26082..26306	+	225	NuclAT_0	-	-
-	26239..26304	-	66	-	-	Antitoxin
L1P11_RS26455	26292..26441	+	150	Protein_38	plasmid maintenance protein Mok	-
L1P11_RS26460	26383..26508	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P11_RS26465	26728..26958	+	231	WP_001426396.1	hypothetical protein	-
L1P11_RS26470	26956..27129	-	174	Protein_41	hypothetical protein	-
L1P11_RS26475	27199..27405	+	207	WP_000547968.1	hypothetical protein	-
L1P11_RS26480	27430..27717	+	288	WP_000107540.1	hypothetical protein	-
L1P11_RS26485	27836..28657	+	822	WP_001234451.1	DUF932 domain-containing protein	-
L1P11_RS26490	28936..29388	-	453	WP_001469955.1	transglycosylase SLT domain-containing protein	-
L1P11_RS26495	29866..30249	+	384	WP_001137956.1	relaxosome protein TraM	-
L1P11_RS26500	30431..31168	+	738	WP_000818288.1	hypothetical protein	-
L1P11_RS26505	31257..31466	+	210	WP_001114348.1	TraY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A)	-	1..82263	82263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T232542 WP_001372321.1 NZ_CP091047:26383-26508 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T232542 NZ_CP127316:c3924353-3923943 [Escherichia coli]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACAGGCCTGATGACACGTAA
TAACTACAGAATGGTAAATAACATTACCCTGGGTAGCATCCGGGGGCGAAACGATGAAACTTTCCCTGACGCTGGAAGCC
GACAGCATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT232542 NZ_CP091047:c26304-26239 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin