Detailed information of TA system TA232538

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73784..74038
• Replicon: plasmid p2_STEC346
• Accession: NZ_CP091046
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: Q0H0B2
• Locus tag: L1P11_RS26210
• Protein ID: WP_001300273.1
• Coordinates: 73889..74038 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	73784..73845 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS26185 (69238)	69238..69984	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
L1P11_RS26190 (70043)	70043..70903	+	861	WP_000704512.1	alpha/beta hydrolase	-
L1P11_RS26195 (71006)	71006..71563	+	558	WP_000139319.1	fertility inhibition protein FinO	-
L1P11_RS26200 (71718)	71718..71921	+	204	WP_001327131.1	hypothetical protein	-
L1P11_RS26205 (72664)	72664..73124	+	461	Protein_96	thermonuclease family protein	-
L1P11_RS26860 (73475)	73475..73609	+	135	WP_001058542.1	hypothetical protein	-
- (73784)	73784..73845	-	62	NuclAT_1	-	Antitoxin
- (73784)	73784..73845	-	62	NuclAT_1	-	Antitoxin
- (73784)	73784..73845	-	62	NuclAT_1	-	Antitoxin
- (73784)	73784..73845	-	62	NuclAT_1	-	Antitoxin
L1P11_RS26210 (73889)	73889..74038	+	150	WP_001300273.1	Hok/Gef family protein	Toxin
L1P11_RS26215 (74322)	74322..74570	+	249	WP_000083838.1	replication regulatory protein RepA	-
L1P11_RS26220 (74643)	74643..74768	-	126	WP_071590472.1	replication protein RepA	-
L1P11_RS26225 (74817)	74817..74891	+	75	WP_001365705.1	RepA leader peptide Tap	-
L1P11_RS26230 (74884)	74884..75741	+	858	WP_001775129.1	incFII family plasmid replication initiator RepA	-
L1P11_RS26235 (76657)	76657..76926	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	-
L1P11_RS26240 (76926)	76926..77204	+	279	WP_248699117.1	type II toxin-antitoxin system toxin YacB	-
L1P11_RS26245 (77250)	77250..77369	+	120	Protein_105	3'-5' exonuclease	-
L1P11_RS26250 (77457)	77457..78395	+	939	Protein_106	IS21-like element IS21 family transposase	-
L1P11_RS26255 (78393)	78393..78554	-	162	Protein_107	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..79133	79133
	-	flank	IS/Tn	-	-	77457..78455	998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5554.72 Da
• Isoelectric Point: 8.7678

>T232538 WP_001300273.1 NZ_CP091046:73889-74038 [Escherichia coli]
MTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T232538 NZ_CP127316:2795503-2795967 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCGATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT232538 NZ_CP091046:c73845-73784 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q0H0B2

Antitoxin