Detailed information of TA system TA232535

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45318..45587
• Replicon: plasmid p2_STEC346
• Accession: NZ_CP091046
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L1P11_RS26035
• Protein ID: WP_001372321.1
• Coordinates: 45462..45587 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45318..45383 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS25985	40367..40612	+	246	WP_071533131.1	hypothetical protein	-
L1P11_RS25990	40605..40940	+	336	WP_014106969.1	hypothetical protein	-
L1P11_RS25995	40853..41059	+	207	WP_000275853.1	hypothetical protein	-
L1P11_RS26000	41085..41624	+	540	WP_000290841.1	single-stranded DNA-binding protein	-
L1P11_RS26005	41687..41920	+	234	WP_000005990.1	DUF905 family protein	-
L1P11_RS26010	41986..43944	+	1959	WP_001775124.1	ParB/RepB/Spo0J family partition protein	-
L1P11_RS26015	43999..44433	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L1P11_RS26020	44430..45192	+	763	Protein_59	plasmid SOS inhibition protein A	-
L1P11_RS26025	45161..45349	-	189	WP_001299721.1	hypothetical protein	-
-	45161..45385	+	225	NuclAT_0	-	-
-	45161..45385	+	225	NuclAT_0	-	-
-	45161..45385	+	225	NuclAT_0	-	-
-	45161..45385	+	225	NuclAT_0	-	-
-	45318..45383	-	66	-	-	Antitoxin
L1P11_RS26030	45371..45520	+	150	Protein_61	plasmid maintenance protein Mok	-
L1P11_RS26035	45462..45587	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P11_RS26040	45807..46037	+	231	WP_086186347.1	hypothetical protein	-
L1P11_RS26045	46035..46208	-	174	Protein_64	hypothetical protein	-
L1P11_RS26050	46278..46484	+	207	WP_000275855.1	hypothetical protein	-
L1P11_RS26055	46509..46796	+	288	WP_000107535.1	hypothetical protein	-
L1P11_RS26060	46914..47735	+	822	WP_001775119.1	DUF932 domain-containing protein	-
L1P11_RS26065	48032..48679	-	648	WP_001775118.1	transglycosylase SLT domain-containing protein	-
L1P11_RS26070	48956..49339	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L1P11_RS26075	49529..50215	+	687	WP_000332492.1	PAS domain-containing protein	-
L1P11_RS26080	50309..50536	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..79133	79133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T232535 WP_001372321.1 NZ_CP091046:45462-45587 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T232535 NZ_CP127316:c2391727-2391320 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGTTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT232535 NZ_CP091046:c45383-45318 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin