Detailed information of TA system TA232517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1163493..1164220
• Replicon: chromosome
• Accession: NZ_CP091044
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: L1P11_RS05745
• Protein ID: WP_000547564.1
• Coordinates: 1163493..1163804 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1P11_RS05750
• Protein ID: WP_000126294.1
• Coordinates: 1163801..1164220 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS05715 (1158635)	1158635..1160344	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
L1P11_RS05720 (1160354)	1160354..1160896	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
L1P11_RS05725 (1160896)	1160896..1161663	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L1P11_RS05730 (1161660)	1161660..1162070	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
L1P11_RS05735 (1162063)	1162063..1162533	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L1P11_RS05740 (1162558)	1162558..1163319	+	762	WP_001026446.1	hypothetical protein	-
L1P11_RS05745 (1163493)	1163493..1163804	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
L1P11_RS05750 (1163801)	1163801..1164220	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L1P11_RS05755 (1164334)	1164334..1165758	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
L1P11_RS05760 (1165767)	1165767..1167224	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L1P11_RS05765 (1167484)	1167484..1168494	+	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
L1P11_RS05770 (1168643)	1168643..1169170	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T232517 WP_000547564.1 NZ_CP091044:1163493-1163804 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T232517 NZ_CP127314:4427503-4427880 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT232517 WP_000126294.1 NZ_CP091044:1163801-1164220 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT232517 NZ_CP127314:4427082-4427456 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5