Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4783549..4784151 Replicon chromosome
Accession NZ_CP091029
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag L1P14_RS23455 Protein ID WP_000897305.1
Coordinates 4783840..4784151 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L1P14_RS23450 Protein ID WP_000356397.1
Coordinates 4783549..4783839 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS23425 (4779475) 4779475..4780377 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
L1P14_RS23430 (4780374) 4780374..4781009 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
L1P14_RS23435 (4781006) 4781006..4781935 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
L1P14_RS23440 (4782265) 4782265..4782507 - 243 WP_001086388.1 protein YiiF -
L1P14_RS23445 (4782726) 4782726..4782944 - 219 WP_001295676.1 CopG family transcriptional regulator -
L1P14_RS23450 (4783549) 4783549..4783839 - 291 WP_000356397.1 NadS family protein Antitoxin
L1P14_RS23455 (4783840) 4783840..4784151 - 312 WP_000897305.1 hypothetical protein Toxin
L1P14_RS23460 (4784380) 4784380..4785288 + 909 WP_001162704.1 alpha/beta hydrolase -
L1P14_RS23465 (4785352) 4785352..4786293 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
L1P14_RS23470 (4786338) 4786338..4786775 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
L1P14_RS23475 (4786772) 4786772..4787644 - 873 WP_000920762.1 virulence factor BrkB family protein -
L1P14_RS23480 (4787638) 4787638..4788237 - 600 WP_001295269.1 glucose-1-phosphatase -
L1P14_RS23485 (4788336) 4788336..4789121 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T232502 WP_000897305.1 NZ_CP091029:c4784151-4783840 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T232502 NZ_CP127314:555793-555900 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT232502 WP_000356397.1 NZ_CP091029:c4783839-4783549 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT232502 NZ_CP127314:c555744-555681 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References