Detailed information of TA system TA023250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1662020..1662582
• Replicon: chromosome
• Accession: NC_011601
• Organism: Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1Q1V8
• Locus tag: E2348C_RS08615
• Protein ID: WP_000605675.1
• Coordinates: 1662304..1662582 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2348C_RS08610
• Protein ID: WP_000781364.1
• Coordinates: 1662020..1662304 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2348C_RS08595 (E2348_C_1608)	1657379..1660426	+	3048	WP_012578903.1	formate dehydrogenase-N subunit alpha	-
E2348C_RS08600 (E2348_C_1609)	1660439..1661323	+	885	WP_001240584.1	formate dehydrogenase N subunit beta	-
E2348C_RS08605 (E2348_C_1610)	1661316..1661969	+	654	WP_000045647.1	formate dehydrogenase-N subunit gamma	-
E2348C_RS08610 (E2348_C_1611)	1662020..1662304	-	285	WP_000781364.1	HigA family addiction module antidote protein	Antitoxin
E2348C_RS08615	1662304..1662582	-	279	WP_000605675.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2348C_RS08620 (E2348_C_1612)	1662768..1663778	-	1011	WP_000642413.1	alcohol dehydrogenase AdhP	-
E2348C_RS08625 (E2348_C_1613)	1663912..1665609	-	1698	WP_000433462.1	malate dehydrogenase	-
E2348C_RS08630 (E2348_C_1614)	1665765..1665902	-	138	WP_000841554.1	stationary-phase-induced ribosome-associated protein	-
E2348C_RS08635 (E2348_C_1615)	1666004..1666219	-	216	WP_000495766.1	biofilm-dependent modulation protein	-
E2348C_RS08640 (E2348_C_1616)	1666564..1666995	+	432	WP_000152307.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10537.16 Da
• Isoelectric Point: 7.3206

>T23250 WP_000605675.1 NC_011601:c1662582-1662304 [Escherichia coli O127:H6 str. E2348/69]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T23250 NC_011601:c1662582-1662304 [Escherichia coli O127:H6 str. E2348/69]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10386.11 Da
• Isoelectric Point: 8.8567

>AT23250 WP_000781364.1 NC_011601:c1662304-1662020 [Escherichia coli O127:H6 str. E2348/69]
MKMANHPRPGDIILESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Nucleotide

• Length: 285 bp

>AT23250 NC_011601:c1662304-1662020 [Escherichia coli O127:H6 str. E2348/69]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCTGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: S1Q1V8