Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3961453..3961994 Replicon chromosome
Accession NZ_CP091029
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag L1P14_RS19650 Protein ID WP_000615976.1
Coordinates 3961716..3961994 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag L1P14_RS19645 Protein ID WP_000729704.1
Coordinates 3961453..3961713 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS19620 (3957236) 3957236..3958021 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
L1P14_RS19625 (3957993) 3957993..3959705 + 1713 Protein_3848 flagellar biosynthesis protein FlhA -
L1P14_RS19630 (3959810) 3959810..3960088 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
L1P14_RS19635 (3960081) 3960081..3960437 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
L1P14_RS19640 (3960494) 3960494..3961267 - 774 WP_000543897.1 C40 family peptidase -
L1P14_RS19645 (3961453) 3961453..3961713 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L1P14_RS19650 (3961716) 3961716..3961994 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L1P14_RS19655 (3962150) 3962150..3962890 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L1P14_RS19660 (3962861) 3962861..3963628 - 768 WP_000333380.1 class II glutamine amidotransferase -
L1P14_RS19665 (3963834) 3963834..3964412 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T232499 WP_000615976.1 NZ_CP091029:3961716-3961994 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T232499 NZ_CP127310:c61381-61229 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT232499 WP_000729704.1 NZ_CP091029:3961453-3961713 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT232499 NZ_CP127310:61436-61493 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References