Detailed information of TA system TA232475

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54358..54784
• Replicon: plasmid p2_STEC383
• Accession: NZ_CP091028
• Organism: Escherichia coli strain STEC383

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L1P12_RS26195
• Protein ID: WP_001372321.1
• Coordinates: 54358..54483 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54560..54784 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P12_RS26150 (49375)	49375..49602	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
L1P12_RS26155 (49739)	49739..50410	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
L1P12_RS26160 (50604)	50604..50987	-	384	WP_235387489.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L1P12_RS26165 (51322)	51322..51912	+	591	WP_165835419.1	transglycosylase SLT domain-containing protein	-
L1P12_RS26170 (52209)	52209..53030	-	822	WP_001775119.1	DUF932 domain-containing protein	-
L1P12_RS26175 (53149)	53149..53436	-	288	WP_000107535.1	hypothetical protein	-
L1P12_RS26180 (53461)	53461..53667	-	207	WP_000275855.1	hypothetical protein	-
L1P12_RS26185 (53737)	53737..53910	+	174	Protein_72	hypothetical protein	-
L1P12_RS26190 (53908)	53908..54138	-	231	WP_086186347.1	hypothetical protein	-
L1P12_RS26195 (54358)	54358..54483	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P12_RS26200 (54425)	54425..54574	-	150	Protein_75	plasmid maintenance protein Mok	-
- (54562)	54562..54627	+	66	NuclAT_0	-	-
- (54562)	54562..54627	+	66	NuclAT_0	-	-
- (54562)	54562..54627	-	66	NuclAT_1	-	-
- (54560)	54560..54784	+	225	NuclAT_0	-	-
- (54560)	54560..54784	-	225	NuclAT_0	-	Antitoxin
- (54560)	54560..54784	-	225	NuclAT_0	-	Antitoxin
- (54560)	54560..54784	-	225	NuclAT_0	-	Antitoxin
- (54560)	54560..54784	-	225	NuclAT_0	-	Antitoxin
L1P12_RS26205 (54596)	54596..54784	+	189	WP_001299721.1	hypothetical protein	-
L1P12_RS26210 (54753)	54753..55515	-	763	Protein_77	plasmid SOS inhibition protein A	-
L1P12_RS26215 (55512)	55512..55946	-	435	WP_042634413.1	conjugation system SOS inhibitor PsiB	-
L1P12_RS26220 (56001)	56001..57959	-	1959	WP_063078168.1	ParB/RepB/Spo0J family partition protein	-
L1P12_RS26225 (58023)	58023..58256	-	234	WP_000005971.1	DUF905 domain-containing protein	-
L1P12_RS26230 (58312)	58312..58842	-	531	WP_032214168.1	single-stranded DNA-binding protein	-
L1P12_RS26235 (58868)	58868..59074	-	207	WP_000547945.1	hypothetical protein	-
L1P12_RS26240 (59102)	59102..59581	+	480	Protein_83	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T232475 WP_001372321.1 NZ_CP091028:c54483-54358 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T232475 NZ_CP127306:332309-332587 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT232475 NZ_CP091028:c54784-54560 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin