Detailed information of TA system TA232467

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34865..35286
• Replicon: plasmid p1_STEC383
• Accession: NZ_CP091027
• Organism: Escherichia coli strain STEC383

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L1P12_RS25355
• Protein ID: WP_096937776.1
• Coordinates: 34865..34990 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35088..35286 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P12_RS25320 (31002)	31002..31640	-	639	WP_000777692.1	type-F conjugative transfer system pilin assembly protein TrbC	-
L1P12_RS25325 (31649)	31649..32641	-	993	WP_000830839.1	conjugal transfer pilus assembly protein TraU	-
L1P12_RS25330 (32638)	32638..33270	-	633	WP_001203720.1	type-F conjugative transfer system protein TraW	-
L1P12_RS25335 (33267)	33267..33653	-	387	WP_000099690.1	type-F conjugative transfer system protein TrbI	-
L1P12_RS25340 (33650)	33650..33967	-	318	Protein_42	hypothetical protein	-
L1P12_RS25345 (33991)	33991..34197	-	207	WP_032219147.1	hypothetical protein	-
L1P12_RS25350 (34267)	34267..34564	+	298	Protein_44	hypothetical protein	-
L1P12_RS25355 (34865)	34865..34990	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P12_RS25360 (34932)	34932..35081	-	150	Protein_46	DUF5431 family protein	-
- (35088)	35088..35286	-	199	NuclAT_0	-	Antitoxin
- (35088)	35088..35286	-	199	NuclAT_0	-	Antitoxin
- (35088)	35088..35286	-	199	NuclAT_0	-	Antitoxin
- (35088)	35088..35286	-	199	NuclAT_0	-	Antitoxin
L1P12_RS25365 (35255)	35255..36017	-	763	Protein_47	plasmid SOS inhibition protein A	-
L1P12_RS25370 (36014)	36014..36447	-	434	Protein_48	conjugation system SOS inhibitor PsiB	-
L1P12_RS25375 (36502)	36502..38460	-	1959	WP_235387474.1	ParB/RepB/Spo0J family partition protein	-
L1P12_RS25380 (38526)	38526..38759	-	234	WP_000005990.1	DUF905 family protein	-
L1P12_RS25385 (38816)	38816..39355	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / tet(A) / sul3	-	1..111805	111805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T232467 WP_096937776.1 NZ_CP091027:c34990-34865 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T232467 NZ_CP127303:c2316507-2316229 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT232467 NZ_CP091027:c35286-35088 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin