Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4060431..4060972 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag L1P12_RS20395 Protein ID WP_000615976.1
Coordinates 4060694..4060972 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag L1P12_RS20390 Protein ID WP_000729704.1
Coordinates 4060431..4060691 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS20355 (4055819) 4055819..4056217 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO -
L1P12_RS20360 (4056220) 4056220..4056513 - 294 WP_059329016.1 type I toxin-antitoxin system antitoxin YafN -
L1P12_RS20365 (4056565) 4056565..4057620 - 1056 WP_053882565.1 DNA polymerase IV -
L1P12_RS20370 (4057691) 4057691..4057915 - 225 Protein_3999 putative lateral flagellar export/assembly protein LafU -
L1P12_RS20375 (4057916) 4057916..4058575 + 660 Protein_4000 flagellar biosynthesis protein FlhA -
L1P12_RS20380 (4058799) 4058799..4059296 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L1P12_RS20385 (4059472) 4059472..4060245 - 774 WP_015675129.1 C40 family peptidase -
L1P12_RS20390 (4060431) 4060431..4060691 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L1P12_RS20395 (4060694) 4060694..4060972 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L1P12_RS20400 (4061128) 4061128..4061868 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L1P12_RS20405 (4061839) 4061839..4062606 - 768 WP_176580751.1 class II glutamine amidotransferase -
L1P12_RS20410 (4062812) 4062812..4063390 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T232465 WP_000615976.1 NZ_CP091026:4060694-4060972 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T232465 NZ_CP127303:1878563-1878913 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT232465 WP_000729704.1 NZ_CP091026:4060431-4060691 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT232465 NZ_CP127303:1878318-1878569 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References