Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4055819..4056513 | Replicon | chromosome |
| Accession | NZ_CP091026 | ||
| Organism | Escherichia coli strain STEC383 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | L1P12_RS20355 | Protein ID | WP_001263493.1 |
| Coordinates | 4055819..4056217 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | - |
| Locus tag | L1P12_RS20360 | Protein ID | WP_059329016.1 |
| Coordinates | 4056220..4056513 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4051479) | 4051479..4051559 | - | 81 | NuclAT_11 | - | - |
| - (4051479) | 4051479..4051559 | - | 81 | NuclAT_11 | - | - |
| - (4051479) | 4051479..4051559 | - | 81 | NuclAT_11 | - | - |
| - (4051479) | 4051479..4051559 | - | 81 | NuclAT_11 | - | - |
| L1P12_RS20325 (4050819) | 4050819..4052063 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| L1P12_RS20330 (4052155) | 4052155..4052613 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| L1P12_RS20335 (4052874) | 4052874..4054331 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| L1P12_RS20340 (4054388) | 4054388..4054909 | - | 522 | Protein_3993 | peptide chain release factor H | - |
| L1P12_RS20345 (4054908) | 4054908..4055111 | - | 204 | Protein_3994 | RtcB family protein | - |
| L1P12_RS20350 (4055357) | 4055357..4055809 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| L1P12_RS20355 (4055819) | 4055819..4056217 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| L1P12_RS20360 (4056220) | 4056220..4056513 | - | 294 | WP_059329016.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| L1P12_RS20365 (4056565) | 4056565..4057620 | - | 1056 | WP_053882565.1 | DNA polymerase IV | - |
| L1P12_RS20370 (4057691) | 4057691..4057915 | - | 225 | Protein_3999 | putative lateral flagellar export/assembly protein LafU | - |
| L1P12_RS20375 (4057916) | 4057916..4058575 | + | 660 | Protein_4000 | flagellar biosynthesis protein FlhA | - |
| L1P12_RS20380 (4058799) | 4058799..4059296 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
| L1P12_RS20385 (4059472) | 4059472..4060245 | - | 774 | WP_015675129.1 | C40 family peptidase | - |
| L1P12_RS20390 (4060431) | 4060431..4060691 | + | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | - |
| L1P12_RS20395 (4060694) | 4060694..4060972 | + | 279 | WP_000615976.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T232464 WP_001263493.1 NZ_CP091026:c4056217-4055819 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T232464 NZ_CP127303:1781608-1781781 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 11263.83 Da Isoelectric Point: 5.9050
>AT232464 WP_059329016.1 NZ_CP091026:c4056513-4056220 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRT
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRT
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT232464 NZ_CP127303:1781284-1781580 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|