Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1193393..1194120 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag L1P12_RS05740 Protein ID WP_235387442.1
Coordinates 1193393..1193704 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L1P12_RS05745 Protein ID WP_000126294.1
Coordinates 1193701..1194120 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS05710 (1188535) 1188535..1190244 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
L1P12_RS05715 (1190254) 1190254..1190796 + 543 WP_000493779.1 formate hydrogenlyase subunit HycF -
L1P12_RS05720 (1190796) 1190796..1191563 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
L1P12_RS05725 (1191560) 1191560..1191970 + 411 WP_096110922.1 formate hydrogenlyase assembly protein HycH -
L1P12_RS05730 (1191963) 1191963..1192433 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
L1P12_RS05735 (1192458) 1192458..1193219 + 762 WP_001026446.1 hypothetical protein -
L1P12_RS05740 (1193393) 1193393..1193704 + 312 WP_235387442.1 type II toxin-antitoxin system HigB family toxin Toxin
L1P12_RS05745 (1193701) 1193701..1194120 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
L1P12_RS05750 (1194234) 1194234..1195658 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
L1P12_RS05755 (1195667) 1195667..1197047 - 1381 Protein_1131 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
L1P12_RS05760 (1197307) 1197307..1198317 + 1011 WP_001402444.1 DNA-binding transcriptional regulator AscG -
L1P12_RS05765 (1198466) 1198466..1198993 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12454.14 Da        Isoelectric Point: 9.7396

>T232450 WP_235387442.1 NZ_CP091026:1193393-1193704 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIRETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T232450 NZ_CP127301:103254-103559 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT232450 WP_000126294.1 NZ_CP091026:1193701-1194120 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT232450 NZ_CP127301:103034-103252 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References