Detailed information of TA system TA232442

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68838..69107
• Replicon: plasmid p_STEC379
• Accession: NZ_CP091025
• Organism: Escherichia coli strain STEC379

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: L1P13_RS25160
• Protein ID: WP_001323520.1
• Coordinates: 68991..69107 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	68838..68903 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P13_RS25120	63986..64132	+	147	Protein_82	adenine-specific DNA-methyltransferase	-
L1P13_RS25125	64183..65409	-	1227	WP_001484257.1	RNA-guided endonuclease TnpB family protein	-
L1P13_RS25130	65468..65872	+	405	WP_001175008.1	IS200/IS605 family transposase	-
L1P13_RS25135	65950..67464	+	1515	Protein_85	ParB/RepB/Spo0J family partition protein	-
L1P13_RS25140	67519..67953	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L1P13_RS25145	67950..68712	+	763	Protein_87	plasmid SOS inhibition protein A	-
L1P13_RS25150	68681..68869	-	189	WP_032084246.1	hypothetical protein	-
-	68681..68905	+	225	NuclAT_0	-	-
-	68681..68905	+	225	NuclAT_0	-	-
-	68681..68905	+	225	NuclAT_0	-	-
-	68681..68905	+	225	NuclAT_0	-	-
-	68838..68903	-	66	-	-	Antitoxin
L1P13_RS25155	68891..69040	+	150	Protein_89	plasmid maintenance protein Mok	-
L1P13_RS25160	68991..69107	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P13_RS25165	69524..69697	-	174	Protein_91	hypothetical protein	-
L1P13_RS25170	69767..69973	+	207	WP_000547968.1	hypothetical protein	-
L1P13_RS25175	69998..70284	+	287	Protein_93	hypothetical protein	-
L1P13_RS25180	70403..71224	+	822	WP_074014913.1	DUF932 domain-containing protein	-
L1P13_RS25185	71521..71807	-	287	WP_235399560.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..71807	71807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T232442 WP_001323520.1 NZ_CP091025:68991-69107 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T232442 NZ_CP127297:4258115-4258393 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT232442 NZ_CP091025:c68903-68838 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin