Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628119..628918 Replicon chromosome
Accession NZ_CP091024
Organism Escherichia coli strain STEC379

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag L1P13_RS03070 Protein ID WP_000347267.1
Coordinates 628119..628583 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L1P13_RS03075 Protein ID WP_001307405.1
Coordinates 628583..628918 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P13_RS03040 (623120) 623120..623554 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L1P13_RS03045 (623572) 623572..624450 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L1P13_RS03050 (624440) 624440..625219 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L1P13_RS03055 (625230) 625230..625703 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L1P13_RS03060 (625726) 625726..627006 - 1281 WP_000681936.1 tagatose-bisphosphate aldolase subunit KbaZ -
L1P13_RS03065 (627255) 627255..628064 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L1P13_RS03070 (628119) 628119..628583 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L1P13_RS03075 (628583) 628583..628918 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L1P13_RS03080 (629067) 629067..630638 - 1572 WP_001273741.1 galactarate dehydratase -
L1P13_RS03085 (631013) 631013..632347 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L1P13_RS03090 (632363) 632363..633133 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T232417 WP_000347267.1 NZ_CP091024:c628583-628119 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T232417 NZ_CP127277:3165253-3165696 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT232417 WP_001307405.1 NZ_CP091024:c628918-628583 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT232417 NZ_CP127277:3165009-3165266 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References