Detailed information of TA system TA232411

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47464..47890
• Replicon: plasmid p_STEC276
• Accession: NZ_CP091023
• Organism: Escherichia coli strain STEC276

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L1O93_RS23925
• Protein ID: WP_001372321.1
• Coordinates: 47464..47589 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47666..47890 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O93_RS23880 (42513)	42513..42740	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
L1O93_RS23885 (42836)	42836..43522	-	687	WP_000332492.1	PAS domain-containing protein	-
L1O93_RS23890 (43712)	43712..44095	-	384	WP_040073112.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L1O93_RS23895 (44417)	44417..45019	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
L1O93_RS23900 (45316)	45316..46137	-	822	WP_074014913.1	DUF932 domain-containing protein	-
L1O93_RS23905 (46256)	46256..46542	-	287	Protein_57	hypothetical protein	-
L1O93_RS23910 (46567)	46567..46773	-	207	WP_000547968.1	hypothetical protein	-
L1O93_RS23915 (46843)	46843..47016	+	174	Protein_59	hypothetical protein	-
L1O93_RS23920 (47014)	47014..47244	-	231	WP_074014914.1	hypothetical protein	-
L1O93_RS23925 (47464)	47464..47589	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1O93_RS23930 (47531)	47531..47680	-	150	Protein_62	plasmid maintenance protein Mok	-
- (47666)	47666..47890	-	225	NuclAT_0	-	Antitoxin
- (47666)	47666..47890	-	225	NuclAT_0	-	Antitoxin
- (47666)	47666..47890	-	225	NuclAT_0	-	Antitoxin
- (47666)	47666..47890	-	225	NuclAT_0	-	Antitoxin
L1O93_RS23935 (47702)	47702..47890	+	189	WP_032084246.1	hypothetical protein	-
L1O93_RS23940 (47859)	47859..48621	-	763	Protein_64	plasmid SOS inhibition protein A	-
L1O93_RS23945 (48618)	48618..49052	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L1O93_RS23950 (49107)	49107..50621	-	1515	Protein_66	ParB/RepB/Spo0J family partition protein	-
L1O93_RS23955 (50699)	50699..51103	-	405	WP_001175008.1	IS200/IS605 family transposase	-
L1O93_RS23960 (51162)	51162..52388	+	1227	WP_001484257.1	RNA-guided endonuclease TnpB family protein	-
L1O93_RS23965 (52439)	52439..52585	-	147	Protein_69	adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	estIa	1..87231	87231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T232411 WP_001372321.1 NZ_CP091023:c47589-47464 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T232411 NZ_CP127277:c3052644-3052228 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT232411 NZ_CP091023:c47890-47666 [Escherichia coli]
TCACATGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin