Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 692017..692744 Replicon chromosome
Accession NZ_CP091022
Organism Escherichia coli strain STEC276

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L1O93_RS03325 Protein ID WP_000550189.1
Coordinates 692017..692331 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L1O93_RS03330 Protein ID WP_001514564.1
Coordinates 692328..692744 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O93_RS03305 (688174) 688174..689160 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
L1O93_RS03310 (689239) 689239..689931 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L1O93_RS03315 (690008) 690008..690511 - 504 WP_001300832.1 M48 family metallopeptidase -
L1O93_RS03320 (690596) 690596..691732 + 1137 WP_001514565.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L1O93_RS03325 (692017) 692017..692331 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L1O93_RS03330 (692328) 692328..692744 + 417 WP_001514564.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L1O93_RS03335 (692789) 692789..694807 - 2019 WP_001544111.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L1O93_RS03340 (695233) 695233..697584 - 2352 WP_240992395.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T232387 WP_000550189.1 NZ_CP091022:692017-692331 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T232387 NZ_CP127277:c1388615-1388322 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT232387 WP_001514564.1 NZ_CP091022:692328-692744 [Escherichia coli]
MITIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT232387 NZ_CP127277:c1388881-1388612 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References