Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 644259..645058 Replicon chromosome
Accession NZ_CP091022
Organism Escherichia coli strain STEC276

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag L1O93_RS03080 Protein ID WP_000347251.1
Coordinates 644259..644723 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L1O93_RS03085 Protein ID WP_001307405.1
Coordinates 644723..645058 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O93_RS03050 (639260) 639260..639694 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L1O93_RS03055 (639712) 639712..640590 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
L1O93_RS03060 (640580) 640580..641359 - 780 WP_029487448.1 PTS N-acetylgalactosamine transporter subunit IIC -
L1O93_RS03065 (641370) 641370..641843 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L1O93_RS03070 (641866) 641866..643146 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L1O93_RS03075 (643395) 643395..644204 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L1O93_RS03080 (644259) 644259..644723 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L1O93_RS03085 (644723) 644723..645058 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L1O93_RS03090 (645207) 645207..646778 - 1572 WP_001273753.1 galactarate dehydratase -
L1O93_RS03095 (647153) 647153..648487 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L1O93_RS03100 (648503) 648503..649273 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T232386 WP_000347251.1 NZ_CP091022:c644723-644259 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T232386 NZ_CP127277:1384172-1384603 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT232386 WP_001307405.1 NZ_CP091022:c645058-644723 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT232386 NZ_CP127277:1383915-1384175 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References