Detailed information of TA system TA232376

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2204493..2204864
• Replicon: chromosome
• Accession: NZ_CP091020
• Organism: Escherichia coli strain STEC308

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: L1O95_RS10880
• Protein ID: WP_021500490.1
• Coordinates: 2204670..2204864 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2204493..2204671 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O95_RS10840 (2199935)	2199935..2200591	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein YobB	-
L1O95_RS10845 (2200693)	2200693..2200923	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
L1O95_RS10850 (2201062)	2201062..2201436	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
L1O95_RS10855 (2201440)	2201440..2202312	+	873	WP_033813010.1	copper homeostasis membrane protein CopD	-
L1O95_RS10860 (2202325)	2202325..2202666	+	342	WP_000976492.1	YebY family protein	-
L1O95_RS10865 (2203059)	2203059..2204135	-	1077	WP_063103093.1	phage integrase Arm DNA-binding domain-containing protein	-
L1O95_RS10870 (2204101)	2204101..2204382	-	282	WP_137689817.1	excisionase	-
- (2204493)	2204493..2204671	+	179	NuclAT_0	-	Antitoxin
- (2204493)	2204493..2204671	+	179	NuclAT_0	-	Antitoxin
- (2204493)	2204493..2204671	+	179	NuclAT_0	-	Antitoxin
- (2204493)	2204493..2204671	+	179	NuclAT_0	-	Antitoxin
L1O95_RS10875 (2204489)	2204489..2204677	-	189	WP_001356607.1	DUF1187 family protein	-
L1O95_RS10880 (2204670)	2204670..2204864	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
L1O95_RS10885 (2204928)	2204928..2205980	-	1053	WP_001004422.1	RecT family recombinase	-
L1O95_RS10890 (2205992)	2205992..2209114	-	3123	WP_063119603.1	exodeoxyribonuclease VIII	-
L1O95_RS10895 (2209216)	2209216..2209491	-	276	WP_000632298.1	hypothetical protein	-
L1O95_RS10900 (2209566)	2209566..2209736	-	171	WP_077633604.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2195284..2268348	73064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T232376 WP_021500490.1 NZ_CP091020:c2204864-2204670 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T232376 NZ_CP127277:717919-718326 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT232376 NZ_CP091020:2204493-2204671 [Escherichia coli]
GATGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin