Detailed information of TA system TA232365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1207811..1208538
• Replicon: chromosome
• Accession: NZ_CP091020
• Organism: Escherichia coli strain STEC308

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: L1O95_RS05900
• Protein ID: WP_000547564.1
• Coordinates: 1207811..1208122 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1O95_RS05905
• Protein ID: WP_000126294.1
• Coordinates: 1208119..1208538 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O95_RS05870 (1202953)	1202953..1204662	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
L1O95_RS05875 (1204672)	1204672..1205214	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
L1O95_RS05880 (1205214)	1205214..1205981	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L1O95_RS05885 (1205978)	1205978..1206388	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
L1O95_RS05890 (1206381)	1206381..1206851	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L1O95_RS05895 (1206876)	1206876..1207634	+	759	Protein_1158	hypothetical protein	-
L1O95_RS05900 (1207811)	1207811..1208122	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
L1O95_RS05905 (1208119)	1208119..1208538	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L1O95_RS05910 (1208652)	1208652..1210076	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
L1O95_RS05915 (1210085)	1210085..1211542	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L1O95_RS05920 (1211802)	1211802..1212812	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
L1O95_RS05925 (1212961)	1212961..1213488	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T232365 WP_000547564.1 NZ_CP091020:1207811-1208122 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T232365 NZ_CP127277:363113-363415 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT232365 WP_000126294.1 NZ_CP091020:1208119-1208538 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT232365 NZ_CP127277:362889-363116 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5