Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 610178..610977 Replicon chromosome
Accession NZ_CP091020
Organism Escherichia coli strain STEC308

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag L1O95_RS02980 Protein ID WP_000347251.1
Coordinates 610178..610642 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L1O95_RS02985 Protein ID WP_001307405.1
Coordinates 610642..610977 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O95_RS02950 (605179) 605179..605613 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L1O95_RS02955 (605631) 605631..606509 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L1O95_RS02960 (606499) 606499..607278 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L1O95_RS02965 (607289) 607289..607762 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L1O95_RS02970 (607785) 607785..609065 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L1O95_RS02975 (609314) 609314..610123 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L1O95_RS02980 (610178) 610178..610642 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L1O95_RS02985 (610642) 610642..610977 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L1O95_RS02990 (611126) 611126..612697 - 1572 WP_001273761.1 galactarate dehydratase -
L1O95_RS02995 (613072) 613072..614406 + 1335 WP_033812942.1 galactarate/glucarate/glycerate transporter GarP -
L1O95_RS03000 (614422) 614422..615192 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T232360 WP_000347251.1 NZ_CP091020:c610642-610178 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T232360 NZ_CP127276:c3692779-3692351 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT232360 WP_001307405.1 NZ_CP091020:c610977-610642 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT232360 NZ_CP127276:c3693025-3692783 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References