Detailed information of TA system TA232337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1207816..1208543
• Replicon: chromosome
• Accession: NZ_CP091018
• Organism: Escherichia coli strain STEC307

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: L1O94_RS05895
• Protein ID: WP_000547564.1
• Coordinates: 1207816..1208127 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1O94_RS05900
• Protein ID: WP_000126294.1
• Coordinates: 1208124..1208543 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O94_RS05865 (1202958)	1202958..1204667	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
L1O94_RS05870 (1204677)	1204677..1205219	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
L1O94_RS05875 (1205219)	1205219..1205986	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L1O94_RS05880 (1205983)	1205983..1206393	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
L1O94_RS05885 (1206386)	1206386..1206856	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L1O94_RS05890 (1206881)	1206881..1207639	+	759	Protein_1157	hypothetical protein	-
L1O94_RS05895 (1207816)	1207816..1208127	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
L1O94_RS05900 (1208124)	1208124..1208543	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L1O94_RS05905 (1208657)	1208657..1210081	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
L1O94_RS05910 (1210090)	1210090..1211547	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L1O94_RS05915 (1211807)	1211807..1212817	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
L1O94_RS05920 (1212966)	1212966..1213493	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T232337 WP_000547564.1 NZ_CP091018:1207816-1208127 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T232337 NZ_CP127276:c2188809-2188513 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT232337 WP_000126294.1 NZ_CP091018:1208124-1208543 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT232337 NZ_CP127276:c2189057-2188806 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5