Detailed information of TA system TA232333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 740163..740856
• Replicon: chromosome
• Accession: NZ_CP091018
• Organism: Escherichia coli strain STEC307

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: L1O94_RS03620
• Protein ID: WP_000415584.1
• Coordinates: 740163..740459 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: L1O94_RS03625
• Protein ID: WP_000650107.1
• Coordinates: 740461..740856 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O94_RS03590 (735838)	735838..736152	-	315	WP_000958598.1	putative quinol monooxygenase	-
L1O94_RS03595 (736183)	736183..736764	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
L1O94_RS03600 (736874)	736874..738223	-	1350	WP_000673406.1	quorum sensing histidine kinase QseC	-
L1O94_RS03605 (738220)	738220..738879	-	660	WP_001221491.1	quorum sensing response regulator transcription factor QseB	-
L1O94_RS03610 (739031)	739031..739423	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
L1O94_RS03615 (739476)	739476..739958	+	483	WP_000183494.1	GyrI-like domain-containing protein	-
L1O94_RS03620 (740163)	740163..740459	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
L1O94_RS03625 (740461)	740461..740856	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
L1O94_RS03630 (740989)	740989..742596	+	1608	WP_001324265.1	ABC transporter substrate-binding protein	-
L1O94_RS03635 (742734)	742734..744992	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rfaE / gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / iroB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH	691941..900254	208313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T232333 WP_000415584.1 NZ_CP091018:740163-740459 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T232333 NZ_CP127276:c1937418-1937029 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCGCGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT232333 WP_000650107.1 NZ_CP091018:740461-740856 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT232333 NZ_CP127276:c1937642-1937415 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65