Detailed information of TA system TA023233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1662552..1663207
• Replicon: chromosome
• Accession: NC_011528
• Organism: Coxiella burnetii CbuK Q154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CBUK_RS08725
• Protein ID: WP_011996617.1
• Coordinates: 1662869..1663207 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBUK_RS08720
• Protein ID: WP_010958263.1
• Coordinates: 1662552..1662863 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUK_RS08700	1657845..1658522	-	678	WP_011996613.1	cyclin protein	-
CBUK_RS08705	1658825..1660207	-	1383	WP_010958260.1	cysteine--tRNA ligase	-
CBUK_RS08710	1660198..1661595	-	1398	WP_005772021.1	glutamate--tRNA ligase	-
CBUK_RS08715	1661751..1662482	+	732	WP_005772022.1	UDP-2,3-diacylglucosamine diphosphatase	-
CBUK_RS08720	1662552..1662863	-	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
CBUK_RS08725	1662869..1663207	-	339	WP_011996617.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBUK_RS08730	1663566..1664711	+	1146	WP_005771875.1	ISAs1-like element ISCbu1 family transposase	-
CBUK_RS08735	1665039..1666712	+	1674	WP_005772026.1	CBU_1493 family Dot/Icm T4SS effector	-
CBUK_RS08740	1666886..1667641	-	756	WP_010958266.1	pyridoxine 5'-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1663566..1664711	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (20-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13428.63 Da
• Isoelectric Point: 10.2262

>T23233 WP_011996617.1 NC_011528:c1663207-1662869 [Coxiella burnetii CbuK_Q154]
MPLTLYIMLDVTRKTVILEVMIKSFKDKYTKYLYEGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T23233 NC_011528:c1663207-1662869 [Coxiella burnetii CbuK_Q154]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATGATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATGAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT23233 WP_010958263.1 NC_011528:c1662863-1662552 [Coxiella burnetii CbuK_Q154]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT23233 NC_011528:c1662863-1662552 [Coxiella burnetii CbuK_Q154]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG