Detailed information of TA system TA232320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2204487..2204858
• Replicon: chromosome
• Accession: NZ_CP091016
• Organism: Escherichia coli strain STEC306

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: L1O96_RS10880
• Protein ID: WP_021500490.1
• Coordinates: 2204664..2204858 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2204487..2204665 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O96_RS10840 (2199929)	2199929..2200585	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein YobB	-
L1O96_RS10845 (2200687)	2200687..2200917	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
L1O96_RS10850 (2201056)	2201056..2201430	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
L1O96_RS10855 (2201434)	2201434..2202306	+	873	WP_033813010.1	copper homeostasis membrane protein CopD	-
L1O96_RS10860 (2202319)	2202319..2202660	+	342	WP_000976492.1	YebY family protein	-
L1O96_RS10865 (2203053)	2203053..2204129	-	1077	WP_063103093.1	phage integrase Arm DNA-binding domain-containing protein	-
L1O96_RS10870 (2204095)	2204095..2204376	-	282	WP_137689817.1	excisionase	-
- (2204487)	2204487..2204665	+	179	NuclAT_0	-	Antitoxin
- (2204487)	2204487..2204665	+	179	NuclAT_0	-	Antitoxin
- (2204487)	2204487..2204665	+	179	NuclAT_0	-	Antitoxin
- (2204487)	2204487..2204665	+	179	NuclAT_0	-	Antitoxin
L1O96_RS10875 (2204483)	2204483..2204671	-	189	WP_001356607.1	DUF1187 family protein	-
L1O96_RS10880 (2204664)	2204664..2204858	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
L1O96_RS10885 (2204922)	2204922..2205974	-	1053	WP_001004422.1	RecT family recombinase	-
L1O96_RS10890 (2205986)	2205986..2209108	-	3123	WP_063119603.1	exodeoxyribonuclease VIII	-
L1O96_RS10895 (2209210)	2209210..2209485	-	276	WP_000632298.1	hypothetical protein	-
L1O96_RS10900 (2209560)	2209560..2209730	-	171	WP_077633604.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2197186..2258060	60874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T232320 WP_021500490.1 NZ_CP091016:c2204858-2204664 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T232320 NZ_CP127276:c754994-754557 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT232320 NZ_CP091016:2204487-2204665 [Escherichia coli]
GATGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin